节律基因hClock及hBmal1对胃癌化疗敏感性的影响

宋俊梅; 邹文萍; 胡欣; 刘康; 李光明

doi:10.3969/j.issn.1000-8179.20130509

节律基因hClock及hBmal1对胃癌化疗敏感性的影响

Influence of circadian genes hClock and hBmal1 on the chemosensitivity of gastric cancer

摘要

摘要:
目的探讨节律基因hClock、hBmal1对胃癌细胞SGC-7901化疗敏感性的影响。
方法体外持续黑暗条件下培养SGC-7901，用实时定量PCR法检测两种主要的节律基因hClock、hBmal1在SGC-7901中不同时间点的表达，分别在节律基因hClock、hBmal1表达的高峰及低谷给予化疗药物多西他赛，CCK-8法检测化疗药物对肿瘤细胞的抑制率。
结果实时定量CR结果显示节律基因hClock、hBmal1在胃癌细胞SGC-7901中不同时间点表达不同，存在时相性波动，表达高峰在20：00，而表达低谷在08：00。CCK-8法显示在节律基因hClock、hBmal1表达高峰时，多西他赛对肿瘤细胞的抑制率低于其在低谷时的抑制率。
结论节律基因hClock、hBmal1过表达可以降低胃癌细胞SGC-7901对多西他赛的敏感性。

Abstract:
Objective To study the influernce of circadian genes hClock and hBmal1 on the chemosensitivity of SGC-7901 cells.
Methods SGC-7901 cells were cultivated under continuous darkness in vitro.The expression levels of the two main circadian genes hClock and hBmal1 at the different time were determined by real-time polymerase chain reaction(PCR). Docetaxel was administered at the peak and nadir time point respectively. The inhibition of SGC-7901 cell proliferation was measured using a CCK-8 kit.
Result The expression of circadian genes hClock and hBmal1 varied at different times, as shown by real-time PCR. The expression of circadian genes hClock and hBmal1 showed Phase oseillation. The maximum expression of hClock and hBmal1 mRNA was at 20:00. whereas their minimum expression was at 08:00. The inhibition ratio of docetaxel to SGC-7901 cells at the maximum expression of hClock and hBmal1 genes was lower than that at the minimum expression.
Conclusion Circadian Genes hClock and hBmal1 can reduce the drug sensitivity of SGC-7901 cell line to docetaxel in vitro.

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