人源电压门控质子通道对乳腺癌细胞迁移与侵袭的影响

王雅蕾; 张尚荣; 王一凡; 孙保存; 李树杰

doi:10.3969/j.issn.1000-8179.20130658

人源电压门控质子通道对乳腺癌细胞迁移与侵袭的影响

Effects of voltage-gated proton channel(Hv1)on the migration and invasion of breast cancer cells

摘要

摘要:
目的明确人源电压门控质子通道蛋白（human voltage-gated proton channel 1，Hv1）对乳腺癌细胞迁移及侵袭能力的影响。
方法检测Hv1在不同转移能力的人乳腺癌细胞系中的表达，利用小RNA干扰（siRNA）技术下调Hv1在乳腺癌MDA-MB-231细胞中的表达，通过细胞划痕和体外侵袭实验方法，观察Hv1对乳腺癌细胞迁移和侵袭的影响并初步探讨相关分子机制。
结果 Hv1在高转移的乳腺癌细胞MDA-MB-231中表达较高，Hv1基因的siRNA干扰片段能够抑制Hv1基因及蛋白的表达；细胞划痕和体外侵袭实验表明Hv1降表达的MDA-MB-231细胞迁移和侵袭能力较弱；明胶酶谱和免疫印迹实验证明下调Hv1基因在MDA-MB-231细胞中的表达明显抑制了MMP-2的活性。
结论 Hv1能够促进乳腺癌细胞迁移及侵袭。

Abstract:
Objective To clarify the effect of voltage-gated proton channel 1 (Hv1) on the migration and invasion of breast cancer cells.
Methods The protein expression of Hv1 was detected in human breast cancer cell lines with different metastatic abilities. SiRNA technique was used to down-regulate the expression of Hvl in breast cancer MDA-MB-231 cells. Scratch and matrigel invasion methods were used to observe the effect of Hvl on the migration and invasion of breast cancer cells, and the relevant molecular mechanism was explored.
Results Hv1 was highly expressed in the highly metastatic breast cancer cell line MDA-MB-231. Hvl was more highly expressed in MDA-MB-231 cells with higher metastatic ability. The SiRNA sequence target at Hvl inhibited Hvl expression. Scratch and matrigel invasion experiments showed that the migration and invasion of MDA-MB-231 cells were significantly attenuated when Hv1 was knocked down by siRNAtargeting Hv1. Zymography experiment on matrix metalloproteinase indicated that the enzyme activities of MMP-2 markedly decreased.
Conclusion Hv1 promoted the migration and invasion ability of breast cancer cells.

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