The expression and clinical significance of B7 family molecules and their receptor PD-1 in human NK/T-cell lymphoma
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摘要:
目的 探讨B7家族及其受体（programmed death-1，PD-1）在人NK/T细胞淋巴瘤（NKTL）中的表达及临床意义。 方法 荧光定量PCR（real time fluorescence quantitative PCR，RQ-PCR）及免疫组织化学SP法检测PD-1及B7家族PD-L1、PD-L2在人NKTL细胞系（SNK-6、YTS）及NKTL组织中的表达，并分析其与临床病理学参数及临床疗效之间的关系。 结果 PD-L1和PD-L2 mRNA在SNK-6、YTS中的表达明显高于正常NK细胞。PD-L1和PD-L2蛋白在NKTL组织中的表达阳性率（分别为60.0%、63.3%）明显高于鼻炎组织（22.2%、16.7%）；NKTL组织中PD-1+肿瘤浸润T淋巴细胞（TIL）数与PD-L1、PD-L2的表达均呈负相关；PD-L1和PD-L2的表达水平与分期均呈正相关，PD-L1与LDH、Ki-67水平呈正相关，PD-L1与IPI评分呈负相关，PD-L1及PD-L2与其他临床病理学参数无显著相关性。PD-L1与治疗疗效呈负相关，而PD-L2与治疗疗效无明显相关性。 结论 B7家族及其受体PD-1在人NKTL中高表达且具有一定的临床意义，PD-L/PD-1信号途径可能参与了NKTL的免疫逃逸，有望成为人NKTL免疫治疗的新靶点。 Abstract:Objective To investigate the expression and clinical significance of B7 family molecules and their receptor PD-1(programmed death-1) in human NK/T-cell lymphoma (NKTL). Methods Real time fluorescence quantitative PCR (RQ-PCR) and Immunohistochemistry SP method were used to detect the expression levels of PD-1 and B7 family molecules (PD-L1 and PD-L2) in human NKTL cell lines (SNK-6 and YTS) and NKTL tissues.The correlation between these proteins and Clinical histopathological parameters and curative effect were analyzed subsequently. Results The expression levels of PD-L1 and PD-L2 mRNA in SNK-6 and YTS were significantly higher than that in normal NK cells.. The expression of PD-L1 and PD-L2 proteins in human NKTL tissues (60.0%、63.3%) and curative effect were significantly increased compared with rhinitis tissues (22.2%、16.7%); furthermore, the mean count of PD-1+Tumor-infiltrating T lymphocytes(TIL) was inversely correlated with PD-L1 and PD-L2 protein expression levels in NKTL tissues.. The expression of PD-L1 and PD-L2 showed a positive correlation with clinical stage.. PD-L1 expression was also positively correlated with LDH, Ki-67 level and was negatively correlated with IPI score. However, there were no significant correlation between PD-L1 and PD-L2 with other Clinical histopathological parameters..-PD-L1 expression was negatively correlated with curative effect. However, there was no correlation between PD-L2 expression and curative effect. Conclusion B7 family molecules and their receptor PD-1 are overexpressed in human NKTL and have greatly clinical significance.PD-L/PD-1 pathway may involved in the tumor immune escape in human NKTL, and it is expected to be a novel target for immunotherapy of human NKTL. -
图 1 SNK-6和YTS中PD-L1、PD-L2 mRNA的相对表达量
Figure 1. The relative expression of PD-L1, PD-L2 mRNA in SNK-6 and YTS cells
图 2 PD-1、PD-L1、PD-L2在NK/T细胞淋巴瘤和鼻炎组织中的表达（SP×200）
Figure 2. The expression of PD-1, PD-L1, PD-L2 in NK/T cell lymphoma and rhinitis tissues(SP method, × 200)
表 1 SNK-6和YTS中PD-L1、PD-L2 mRNA的相对表达量（x±s）
Table 1. The relative expression of PD-L1, PD-L2 mRNA in SNK-6 and YTS cells(x±s)
表 2 PD-L1、PD-L2的表达水平与PD-1+淋巴细胞浸润程度的相关性
Table 2. The correlation between PD-L1, PD-L2 expression level and PD-1+ lymphocyte infiltration
表 3 NKTL组织中PD-L1、PD-L2蛋白的表达与临床病理学参数的关系
Table 3. The relationship between PD-L1, PD-L2 protein expression of NKTL tissues and clinicopathological parameters
表 4 PD-L1和PD-L2基因表达与NKTL治疗疗效的相关性
Table 4. The correlation between PD-L1, PD-L2 expression and curative effect of NKTL
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