伍娇娇, 冯定庆, 田园, 徐汉杰, 李兵, 凌斌. 组蛋白乙酰化修饰调控RAR-β2表达与宫颈病变的相关性[J]. 中国肿瘤临床, 2014, 41(5): 300-304. DOI: 10.3969/j.issn.1000-8179.20131140
引用本文: 伍娇娇, 冯定庆, 田园, 徐汉杰, 李兵, 凌斌. 组蛋白乙酰化修饰调控RAR-β2表达与宫颈病变的相关性[J]. 中国肿瘤临床, 2014, 41(5): 300-304. DOI: 10.3969/j.issn.1000-8179.20131140
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WU Jiaojiao, FENG Dingqing, TIAN Yuan, XU Hanjie, LI Bing, LING Bin. Correlation between cervical lesion development and histone acetylation modification that regulates RAR-β2 expression[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2014, 41(5): 300-304. DOI: 10.3969/j.issn.1000-8179.20131140
Citation: WU Jiaojiao, FENG Dingqing, TIAN Yuan, XU Hanjie, LI Bing, LING Bin. Correlation between cervical lesion development and histone acetylation modification that regulates RAR-β2 expression[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2014, 41(5): 300-304. DOI: 10.3969/j.issn.1000-8179.20131140
shu

组蛋白乙酰化修饰调控RAR-β2表达与宫颈病变的相关性

Correlation between cervical lesion development and histone acetylation modification that regulates RAR-β2 expression

    摘要
  • 摘要:
      目的  探讨组蛋白乙酰化修饰与RAR-β2表达及宫颈病变发生发展的关系。
      方法  收集正常宫颈、宫颈上皮内瘤变（cervical intraepithelial neoplasia，CIN）Ⅱ~Ⅲ和宫颈鳞状细胞癌（squamous cell carcinoma，SCC）组织，分别采用免疫组织化学和Western Blot方法检测AcH3、RAR-β2和Involucrin的表达；分析组蛋白乙酰化水平与RAR-β2表达及宫颈病变程度的关系。
      结果  随着宫颈病变的进展，AcH3、RAR-β2和Involucrin的表达逐渐降低，甚至缺失，组间差异有统计学意义（P＜0.05）；组蛋白乙酰化水平与RAR-β2表达呈正相关（r=0.797，P＜0.05），AcH3和RAR-β2的表达均与宫颈病变程度呈负相关r=-0.547（AcH3），r=-0.585（RAR-β2），P＜0.05。
      结论  组蛋白乙酰化修饰与RAR-β2表达调控有关，并可能参与宫颈癌的发生。

     

    Abstract:
      Objective  To investigate the relationship between cervical lesion development and histone acetylation that regulates RAR-β2 expression.
      Methods  Immunohistochemistry and Western blot analysis were performed to detect AcH3, RAR-β2, and involucrin expression in normal cervical tissues as well as in tissues with cervical intraepithelial neoplasia (CIN) Ⅱ-Ⅲ and squamous cell cervical carcinoma. The relationship among histone acetylation level, RAR-β2 expression, and cervical lesion severity were analyzed.
      Results  AcH3, RAR-β2, and involucrin expression were reduced or absent with the progression of cervical lesions; significant differences were noted between the groups (P < 0.05). Histone acetylation level and RAR-β2 expression were positively correlated (r=0.797, P < 0.05). AcH3 and RAR-β2 expression, which were both associated with the cervical lesions, were negatively correlated r=-0.547(AcH3), r=-0.585(RAR-β2), P < 0.05.
      Conclusion  Histone acetylation modification is associated with the regulation of RAR-β2 expression. This process is also likely to participate in the carcinogenesis of cervical carcinoma.

     

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