Expression of XIAP and Smac in human non-small-cell lung carcinoma(NSCLC)and the relationship with clinical significance and prognosis
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摘要:
目的 探讨XIAP（X-linked inhibitor of apoptosis protein，XIAP）和Smac（second mitochondria-derived activator of caspase，Smac）在非小细胞肺癌（non-small cell lung cancer，NSCLC）组织中的表达与临床病理特征及预后的关系。 方法 采用免疫组织化学法检测70例非小细胞肺癌组织及70例对应癌旁肺组织中XIAP、Smac的表达。 结果 XIAP在70例NSCLC组织中有59例阳性表达，其中高表达16例；对应70例癌旁肺组织中有52例表达，其中高表达5例，两组XIAP表达强度比较差异有统计学意义（Z=-4.049，P < 0.001）；Smac在70例肺癌组织中有63例阳性表达，其中高（强阳性）表达32例；对应70例癌旁肺组织有53例表达，其中高（强阳性）表达5例，两组Smac表达强度比较差异有统计学意义（Z=-5.484，P < 0.001）。NSCLC组织中XIAP、Smac的表达与患者的性别、年龄、肿瘤大小、组织类型、分化程度、吸烟与否等无明显关系（P <0.05）；但二者的表达均与临床分期、淋巴结转移与否有关系（P < 0.05）。通过Kaplan-Meier法分析得出，XIAP和Smac在NSCLC中的表达与患者的预后均无明显关系（P <0.05）。 结论 1）XIAP和Smac在非小细胞肺癌组织及其对应癌旁肺组织中均有表达，但存在表达量的差异。2）XIAP和Smac在非小细胞肺癌中的表达与患者的预后均无显著关系。 -
关键词:
- X连锁凋亡抑制蛋白 /
- 第二个线粒体衍生的半胱天冬酶激活蛋白 /
- 非小细胞肺癌 /
- 免疫组化 /
- 预后
Abstract:Objective To investigate the expression of XIAP and Smac in human non-small-cell lung carcinoma (NSCLC) and the relationship with clinical significance and prognosis. Methods Immunohistochemical staining was performed to determine the expression of X-linked inhibitor of apoptosis protein (XIAP) and second mitochondria-derived activator of caspase (Smac) in 70 cases of NSCLC and 70 cases of non-cancerous adjacent lung tissues. Results XIAP is mostly present (59/70) in tumor tissues with 16 high expressions, whereas only five high expressions in non-cancerous adjacent lung tissues are observed (52/70). The statistical difference of these two sets of data is significant (Z=-5.484, P < 0.001). Comparatively, Smac is present (63/70) in tumor tissues, which is significantly (Z=-5.484, P < 0.001) higher than in the non-cancerous adjacent lung tissues (53/70). The expression levels of XIAP and Smac in NSCLC tissues are closely related to the lymph node metastasis at the TNM stages (P < 0.05) and not associated to gender, age, size of tumor, and differentiation grades (P <0.05). The Kaplan-Meier analysis results show that survival by XIAP and Smac protein in NSCLC has no significant effect (P <0.05). Conclusion XIAP and Smac are expressed in NSCLC and noncancerous adjacent lung tissues, and the differences in their expression levels is significant. The deterioration of NSCLC results in apoptosis/anti-apoptotic synchronized with tumor cell proliferation. The expression levels of XIAP and Smac in NSCLC are not related with the prognosis. -
图 1 XIAP在肺鳞癌组织阳性表达（Elivision×200）
Figure 1. Positive expression of XIAP in squamous cell carcinoma of lung (Elivision×200)
图 2 XIAP在肺腺癌组织阳性表达（Elivision×200）
Figure 2. Positive expression of XIAP in lung adenocarcinoma cell (Elivision×200)
图 3 Smac在肺鳞癌组织阳性表达（Elivision×200）
Figure 3. Positive expression of Smac in squamous cell carcinoma of lung (Elivision×200)
图 4 Smac在肺腺癌组织阳性表达（Elivision×200）
Figure 4. Positive expression of Smac in lung adenocarcinoma cell (Elivision×200)
图 5 肺癌XIAP的高表达和低表达组的生存曲线比较
Figure 5. Comparison of the survival curves of XIAP's high expression and low-expression group in human non-small-cell lung carcinoma(NSCLC)
图 6 肺癌Smac的高表达和低表达组的生存曲线比较
Figure 6. Comparison of the survival curves of Smac's high expression and low-expression group in human non-small-cell lung carcinoma(NSCLC)
表 1 癌组织与癌旁肺组织中XIAP表达强度比较
Table 1. Expression of XIAP in tumor tissues and non-cancerous adja cent lung tissues
表 2 NSCLC组织与癌周肺组织中Smac表达强度比较
Table 2. Expression of Smac in tumor tissues and non-cancerous adjacent lung tissues Tissues
表 3 XIAP和Smac在肺癌中的表达与临床病理参数的关系（续表 3）
Table 3. Expression of XIAP and Smac in human non-small lung cancer(NSCLC)and its relationship with clinical significance
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