EZH2和Ki-67蛋白在腺样囊性癌中的表达及其临床意义

褚金曼; 杜咏梅; 骆树瑜; 李蕊; 董瑞

doi:10.3969/j.issn.1000-8179.20131675

EZH2和Ki-67蛋白在腺样囊性癌中的表达及其临床意义

Expression of EZH2 and Ki-67 in salivary adenoid cystic carcinoma and their clinical significance

摘要

摘要:
目的研究人涎腺腺样囊性癌组织中EZH2（enhancer of zeste homolog2）和Ki-67的表达及其相关性。
方法采用免疫组织化学染色法，检测石蜡包埋的42例涎腺腺样囊性癌（salivary adnoid cystic carcinoma，SACC）及5例正常涎腺组织中，多梳组蛋白EZH2和细胞周期蛋白Ki-67的表达水平，分析与临床病理特征之间的关系并探讨二者的相关性。
结果 EZH2在腺样囊性癌组织中的表达显著高于正常涎腺组织（P＜0.05），EZH2表达阳性率66.67%（28/42），EZH2表达与肿瘤病理分型及临床分期相关，而与性别、年龄、发病部位不相关，而在5例正常涎腺组织中不表达。在Ki-67阳性的33例腺样囊性癌患者中，25例EZH2表达阳性，表达率75.76%（25/33），Ki-67阴性表达的9例中，3例EZH2表达阳性，表达率33.33%（3/9），两者比较差异有统计学意义（P＜0.05）。
结论 EZH2在腺样囊性癌中表达增加且与肿瘤细胞的增殖密切相关，提示腺样囊性癌中EZH2通过其在细胞周期管理中的作用，参与肿瘤细胞的增殖过程。

Abstract:
Objective This study aimed to investigate the expressions of EZH2 and Ki-67 in the salivary adenoid cystic carcinoma (SACC) of humans and their correlation.
Methods A total of 42 cases of SACC tumor tissues and 5 cases of normal tissues were considered to determine the expressions of EZH2 and Ki-67 by immunohistochemistry. The relationship and correlation of such expressions with the clinicopathological characteristics were also analyzed.
Results The expression of EZH2 was notably higher in SACC than in normal tissues (P < 0.05). EZH2 expression was detected in 66.67% (28/42) of the tumor tissues. This expression was correlated with pathological grade and clinical stage. By contrast, EZH2 expression did not correlate with gender, age, and localization. EZH2 was not expressed in normal tissues. The incidence of EZH2 expression in the Ki-67 positive group was 75.76% (25/33) and the incidence in the Ki-67 negative group was 33.33% (3/9). The difference between the two groups was statistically significant (P < 0.05).
Conclusion The increased expression of EZH2 in SACC was related to tumor proliferation. EZH2 may participate in tumor cell proliferation via cell cycle management.

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