Abstract:
Objective To detect the expression of miR-106b~25 cluster in glioma cell line and tissues.
Methods Real-time PCR was performed to determine the expression of miR-106b~25 cluster members (miR-106b, miR-93, and miR-25) in different human glioblastoma cell lines. Different pathological grade glioma specimens were surgically removed. In-situ hybridization was performed to detect the expression of miR-106b~25 cluster members in different pathological levels of glioma tissues.
Results In the expression of the benchmark on normal brain tissues, three kinds of miRNAs in all test cell lines have a tendency to increase. Based on the expression of the pathological level Ⅰ average rate in 43 cases of glioma specimens collected after neurosurgical operations, the real-time PCR results showed that the average expression quantity of the three kinds of miRNAs in each group gradually increase. The increase in tumor pathological levels results in statistically significant expression differences of miR-106b and miR-93 between the groups (F=4.479, P= 0.018 and F=3.493, P=0.040, respectively). However, miR-25 expression differences between the groups have no statistically significant differences (F=2.766, P=0.075). In situ hybridization results show that the expressions of three miRNAs in high grade gliomas are significantly higher than that in the low-level tumor. Spearman rank correlation analysis results indicate that the expression of these miRNAs signal-intensity distribution is positively correlated with glioma, in accordance with WHO pathology classification. The correlation coefficient for miR-106b, miR-93, and miR-25 are 0.617, 0.438, and 0.463, respectively (P < 0.001).
Conclusion The expression of miR-106b~25 cluster members is up-regulated in the glioma and is positively correlated with tumor grade.