活体自发光成像监测长春瑞滨纳米胶束抗乳腺癌转移机制研究

张发云; 秦蕾; 梁伟

doi:10.3969/j.issn.1000-8179.20131858

活体自发光成像监测长春瑞滨纳米胶束抗乳腺癌转移机制研究

Monitoring anti-metastasis effect of vinorelbine-encapsulated micelles on breast cancer by bioluminescence imaging

摘要

摘要:
目的乳腺肿瘤细胞的转移是乳腺癌致死的主要原因。淋巴转移作为血道和淋巴两大转移途径之一，在乳腺癌转移过程中至关重要。针对乳腺癌转移的特点，本研究组设计了具有淋巴富集特性的纳米载药系统，活体动态监测其对原位乳腺肿瘤生长以及肺脏和淋巴器官转移的抑制效果，旨在为临床用药提供科学依据。
方法采用活体自发光成像实时动态地监测了聚乙二醇化磷脂（PEG-PE）包载的长春瑞滨纳米胶束抗乳腺癌体内转移的过程。
结果与未包载的游离长春瑞滨（Free Vin）相比，PEG-PE包载的长春瑞滨胶束（NanoVin）增强了长春瑞滨的抗原位瘤活性，显著抑制了乳腺肿瘤细胞的淋巴及肺脏转移。
结论通过静脉给药达到了血道转移和淋巴转移两条途径的双重清扫，为靶向药物设计提供了新思路。活体光学成像技术可动态监测肿瘤的转移，为临床应用影像技术检测药物疗效提供必要的技术手段。

Abstract:
Objective Breast cancer metastasis is a major cause of death. Lymphatic metastasis is one of the two main metastatic ways that is crucial to the metastasis process of breast cancer. To treat breast cancer metastasis, we prepared micelle-based drug carrier for lymphatic delivery.
Methods We used in vivo bioluminescence imaging for real-time dynamic monitoring of the inhibitory effect of polyethylene glycol phospholipid micelle encapsulating vinorelbine on breast cancer metastasis.
Results Compared with the free vinorelbine, vinorelbine-encapsulated micelles (NanoVin) showed a significantly enhanced anti-tumor activity both in primary and metastatic tumors.
Conclusion Intravenous administration of NanoVin markedly prevented the metastasis of tumor cells through both hematogenous and lymphatic systems. This study provides a new approach for treatment of metastatic tumors. Bioluminescence imaging is a powerful tool to dynamically monitor tumor metastasis and clinical therapeutic effect of anticancer drugs.

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