胃癌组织EB病毒感染与miR-101EZH2 COX-2表达关系的研究

袁浩; 刘伟; 姜楠; 王玉平; 陈兆峰; 刘敏; 周永宁

doi:10.3969/j.issn.1000-8179.20131988

胃癌组织EB病毒感染与miR-101EZH2 COX-2表达关系的研究

doi: 10.3969/j.issn.1000-8179.20131988

袁浩^{①, ③,},
刘伟^②,
姜楠^③,
王玉平^{①, ③},
陈兆峰^{①, ③},
刘敏^{①, ③},
周永宁^{①, ③, ,}

①.
兰州大学第一医院消化内科
②.
兰州大学基础医学院
③.
甘肃省胃肠病重点实验室（兰州市 730000）

基金项目:

国家自然科学基金项目 81372145

甘肃省卫生行业科研计划项目 GSWST09-03

详细信息

作者简介:
袁浩硕士研究生。研究方向为消化系统肿瘤。E-mail：yh230987@126.com

通讯作者:
周永宁 yongningzhou@sina.com

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- 被引次数: 0
出版历程
- 收稿日期: 2013-11-21
- 修回日期: 2014-01-03
- 刊出日期: 2014-04-15

Correlation of Epstein-Barr virus infection with the expression of miR-101, EZH2, and COX-2 in gastric cancer

YUAN Hao^{①, ③
,},
LIU Wei^②,
JIANG Nan^③,
WANG Yuping^{①, ③},
CHEN Zhaofeng^{①, ③},
LIU Min^{①, ③},
ZHOU Yongning^{①, ③
, ,}

①.
The First Clinical Medical College of Lanzhou University; Department of Gastroenterology, The First Hospital of Lanzhou University
②.
School of Basic Medical Sciences
③.
Gansun Provincial Key Laboratory of Gastroenteropathy; Lanzhou University, Lanzhou 730000, China

Funds:

the National Natural Science Foundation of China 81372145

Management Project of Gansu Provincial Health Scientific Research Plan GSWST09-03

More Information

Corresponding author: Yongning ZHOU; E-mail: yongningzhou@sina.com

摘要

摘要: 目的探讨甘肃省武威地区胃癌患者EB病毒（Epstein-Barr virus，EBV）感染状况及miR-101、EZH2、COX-2在EBV相关胃癌发生发展中的作用。方法应用组织芯片、原位杂交和免疫组织化学技术检测120例胃癌组织及相应癌旁组织中EBV小RNA（EBER）、miR-101、EZH2、COX-2的表达情况。结果 120例胃癌组织EBV阳性率为10.0%，EBV相关胃癌有较少的淋巴结转移，好发于贲门、胃体（P < 0.05）。miR-101、EZH2、COX-2在120例胃癌组织和相应癌旁组织的阳性率差异有统计学意义（P < 0.05）。12例EBV阳性胃癌组织miR-101、EZH2、COX-2和108例EBV阴性胃癌组织3个分子的表达率差异有统计学意义（P < 0.05）。胃癌组织EBV感染和miR-101表达呈正相关，EBV相关胃癌组织miR-101表达和淋巴结转移、COX-2、EZH2表达均呈负相关（P < 0.05）。结论 EB病毒感染与武威地区胃癌的发生有一定关系；EBV相关胃癌和EBV阴性胃癌在淋巴结转移和发生部位的差异有统计学意义；miR-101、EZH2、COX-2与EB病毒相关胃癌的发展有一定关系。
- EB病毒 /
- 胃癌 /
- 原位杂交 /
- miR-101
Abstract: Objective This study was conducted to investigate Epstein-Barr virus (EBV) infection in gastric cancer in Wuwei area of Gansu province (China) and the roles of miR-101, EZH2, and COX-2 in EBV-associated gastric carcinomas (EBVaGC). Methods Tissue microarray technique, immunohistochemistry, and in situ hybridization were performed to detect the expression of EBV-encoded small RNAs (EBERs), miR-101, EZH2, and COX-2, in gastric cancer tissues (n=120) and the corresponding adjacent tissues (n= 120). Results The positive rate of EBV was 10.0% in 120 cases of gastric cancer tissues. EBVaGC was not significantly associated with lymph node metastasis and developed most often in the cardia and body (P<0.05). The differences in the positive rates of miR-101, EZH2, and COX-2 in 120 cases of gastric cancer tissues and corresponding adjacent tissues were significant (P<0.05). The differences in the positive rates of miR-101, EZH2, and COX-2 in 12 cases of EBVaGC and in 108 cases of EBV-negative gastric cancer (EBVnGC) tissues were significant (P<0.05). The infection of EBV in gastric cancer tissues was positively related to miR-101 expression. By contrast, miR-101 expression was negatively related to lymph node metastasis and expression of COX-2 and EZH2 in EB-VaGC tissues (P<0.05). Conclusion EBV infection was related to gastric cancer in Wuwei area of Gansu province. EBVaGC and EB-VnGC have significant differences in lymph node metastasis and in the location of cancer. MiR-101, EZH2, and COX-2 were related to the development of EBVaGC.
- in situ hybridization /
- gastric cancer /
- miR-101 /
- <

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图 1 EBER、miR-101、EZH2、COX-2在EBVaGC和EBVnGC中的表达

Figure 1. Expression of EBER、miR-101、EZH2、COX-2 in EBVaGC and EBVnGC

A. EBER expression in EBVaGC（ISH×200）；B. miR-101 expression in EBVaGC（ISH×200）；C. EZH2 expression in EBVnGC（IHC×200）；D. COX-2 expression in EBVnGC（IHC×200）

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表 1 EBVaGC和EBVnGC患者的临床病理特征

Table 1. Clinicopathological parameters of EBVaGC and EBVnGC

表 2 miR-101、EZH2、COX-2在癌、癌旁、EBVaGC、EBVnGC中的表达

Table 2. Expression of miR-101, EZH2, and COX-2 in cancer tissues, adjacent tissues, EBVaGC, and EBVnGC

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