王红艳, 郑少秋, 涂永生, 张雅洁. 肺癌转移相关microRNA miR-29b的生物信息学分析*[J]. 中国肿瘤临床, 2014, 41(16): 1021-1025. DOI: 10.3969/j.issn.1000-8179.20132018
引用本文: 王红艳, 郑少秋, 涂永生, 张雅洁. 肺癌转移相关microRNA miR-29b的生物信息学分析*[J]. 中国肿瘤临床, 2014, 41(16): 1021-1025. DOI: 10.3969/j.issn.1000-8179.20132018
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WANG Hongyan, ZHENG Shaoqiu, TU Yongsheng, ZHANG Yajie. Bioinformatics analysis of lung cancer metastasis-related miR-29b[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2014, 41(16): 1021-1025. DOI: 10.3969/j.issn.1000-8179.20132018
Citation: WANG Hongyan, ZHENG Shaoqiu, TU Yongsheng, ZHANG Yajie. Bioinformatics analysis of lung cancer metastasis-related miR-29b[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2014, 41(16): 1021-1025. DOI: 10.3969/j.issn.1000-8179.20132018
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肺癌转移相关microRNA miR-29b的生物信息学分析*

Bioinformatics analysis of lung cancer metastasis-related miR-29b

    摘要
  • 摘要: 目的： 应用生物信息学分析A549细胞中在CD133阳性低表达的miR-29b的靶基因及其功能，为以miR-29b为靶点的肿瘤研究提供线索。 方法： 利用miRNA PCR芯片筛选A549细胞中CD133阳性和CD133阴性差异表达的miRNA，选用miRecords预测miR-29b的靶基因，合并已证实的靶基因，利用GOEAST和DAVID数据库对所得靶基因进行功能富集分析和信号转导通路富集分析。 结果： A549细胞中与CD133阴性比较，miR-29b在CD133阳性中表达下调。miR-29b靶基因有106个，其靶基因功能富集于结合和细胞外基质形成等作用（P<0.01）；信号转导通路显著富集于JAK-STAT和TGF-β等信号转导通路（P<0.05）。 结论： miR-29b可能与肺癌转移相关，miR-29b的靶基因显著富集在与肿瘤相关的信号通路中。

     

    Abstract: Objective: This paper aims to bioinformatically analyze the target genes of miR-29b and to provide clues for cancerresearch targeting miR-29b. Methods: The differential expression levels of miRNAs in CD133+ and CD133- A549 cells were detectedusing the miRNA PCR chip. Real-time polymerase chain reaction was performed to verify the partially differential expression of miRNAs. Target genes of miR-29b were predicted by miRecords and analyzed by gene ontology and signal transduction pathway enrichment analysis. Results: The miR-29b expression was significantly decreased in the CD133 + A549 cells compared with that in theCD133- cells. The number of miR-29b target genes was 106. The functions of these target genes were enriched in binding and extracellular matrix structural constituent (P<0.01). The JAK-STAT and TGF-β signal transduction pathways were significantly enriched (P＜0.05). Conclusion: The abnormal expression of miR-29b may be related to metastasis. Some of the predicted target genes of miR-29bwere significantly enriched in the signaling pathways in relation to the tumors.

     

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