Abstract:
Objective To examine the oncogenic mutations involved in melanoma in Southern China and to provide a theoretical basis for the development of melanoma molecular targeted therapy strategy.
Methods The Sequenom platform (OncoCarta Panel v1.0 and MassARRAY System) was used to determine the prevalence of oncogene mutations in 28 acral melanoma samples, 28 mucosal melanoma samples, and 30 non-chronic sun-induced-damage (no-CSD) melanoma samples from Southern China.
Results At least one mutation was detected in 33 of the 86 melanomas (38.4%) with mutations observed in BRAF (16.3%), NRAS (10.5%), KIT (5.8%), EGFR (4.7%), HRAS (2.3%), KRAS (2.3%), MET (2.3%), and PIK3CA (1.2%).In BRAF, the age of patients with mutations was significantly lower than those without BRAF mutation (45.7±15.3 vs.55.9±12.7, P=0.01).Patients with mutations in NRAS were more likely to have ulceration compared with patients without NRAS mutations (88.9% vs.48.1%, P=0.049).
Conclusions This study represents a comprehensive and concurrent analysis of the major recurrent oncogenic mutations involved in melanoma cases from Southern China areas. The data have implications for both clinical trial designs and therapeutic strategies.