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摘要:
目的 观察黄芩素对人乳腺癌MDA-MB-231细胞SATB1蛋白表达的影响。 方法 用MTT法、划痕愈合实验观察不同浓度黄芩素干预后 MDA-MB-231 细胞增殖和运动迁移能力的变化；用 Western Blot 法检测黄芩素干预后 MDA-MB-231 细胞SATB1蛋白表达的变化。 结果 随作用时间的延长和药物浓度的增加，黄芩素对MDA-MB-231细胞增殖和运动迁移的抑制作用逐渐增强，呈明显的时间-剂量依赖性（P＜0.05）；黄芩素可显著降低MDA-MB-231细胞中SATB1蛋白的表达，而且随着药物浓度增加，SATB1蛋白表达量逐渐减少（P＜0.05）。 结论 黄芩素可通过抑制SATB1的表达抑制肿瘤细胞的恶性增殖、侵袭和迁移能力。 Abstract:Objective To investigate the effect of Baicalein on SATB1 expression and its relation to proliferation, invasivenessand migration of MDA-MB-231 cells. Methods Colorimetric 3-(4, 5-dimethylthiazol-2-yl) 2, 5-diphenyltetrazolium bromide (MTT)and wound healing assays were used to detect the cell proliferation and the migration changes of MDA-MB-231 cells when treated withdifferent concentrations of Baicalein. SATB1 expression in MDA-MB-231 cells was detected by Western blot. Results Along with thereaction time and increase drug concentration, the inhibitory effect of Baicalein on proliferation and migration of MDA-MB-231 cellsgradually increased in a time- and dose- dependent manner (P＜0.05). After treatment with Baicalein for 48 h, an obvious decrease wasobserved in the level of SATB1 protein expression of MDA-MB-231 cells in a dose-dependent manner (P＜0.05). Conclusion Baicalein can suppress MDA-MB-231 cells growth and migration by inhibiting its proliferation in a time- and dose- dependent manner. It canalso down-regulate the expression of SATB1. -
Key words:
- Baicalein /
- SATB1 /
- breast cancer /
- MDA-MB-231 cells
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图 2 黄芩素对MDA-MB-231细胞的时间-剂量增殖抑制曲线
Figure 2. Inhibition of MDA-MB-231 cells proliferation by Baicalein in a time- and dose-dependent manner （x±s，n=6）
图 3 黄芩素对MDA-MB-231细胞迁移能力的影响（现宽度/原宽度，x±s，n=6）。24h 组：对照组 vs.10 μM 组 P=0.051，vs. 20 μM 组 P＜0.05；48h组：对照组vs.10 μM组P＜0.01，vs.20 μM组P＜0.01. 采用单因素方差分析，*P＜0.05，**P＜0.01。
Figure 3. Effect of Baicalein on MDA-MB-231 cell migration in vitro 40×（Current width/Original width，x±s，n=6）.（a）Photographs of wound cells treated with Baicalein in different concentration at 0，24，and 48 h.（b）Quantification of the wound healing assay. *P＜0.05 compared with control；**P＜0.01 compared with control，one-way ANOVA test
图 4 Western Blot检测黄芩素对MDA-MB-231细胞中SATB1蛋白表达的影响
A：Western blot analysis of Baicalein in the down-regulation of SATB1 expression in MDA-MB-231 cells. The results show that Baicalein could inhibit the SATB1 expression in a dose-dependent manner. B：Quantification of the western blot assay. *P<0.05，**P<0.01，one-way ANOVA test
Figure 4. Effect of Baicalein on the SATB1 expression in MDA-MB-231 cells
表 1 黄芩素对人乳腺癌MDA-MB-231细胞增殖的影响（x±s，n=6）
Table 1. Inhibitory effects of Baicalein on MDA-MB-231 cell migration（x±s，n=6）
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