氢生理盐水对大鼠缺血再灌注肝细胞线粒体功能的保护作用

李宝山; 刘渠; 李强

doi:10.3969/j.issn.1000-8179.2014.02.20131465

氢生理盐水对大鼠缺血再灌注肝细胞线粒体功能的保护作用

Protective effect of hydrogen-rich saline on liver mitochondrial injury in rats with ischemia-reperfusion

摘要

摘要:
目的研究氢生理盐水（HS）对大鼠缺血再灌注肝细胞线粒体功能的保护作用并探讨其可能机制。

方法将雄性SD大鼠随机分为3组（n=14），除假手术组外，均采用阻断左中肝蒂造成缺血再灌注模型，不同组大鼠分别给予等量生理盐水和HS（5 ml/Kg，i.p.），再灌注180min后分别检测其线粒体功能及线粒体MDA、GSH/GSSH变化情况，电镜观察各组大鼠肝细胞线粒体的形态学改变。

结果缺血再灌注后，大鼠线粒体功能出现明显损伤，HS组肝细胞线粒体功能及线粒体氧化损伤程度明显轻于对照组（P < 0.01）。电镜观察缺血再灌注后，肝细胞线粒体出现明显损伤，HS组病理改变明显轻于对照组。

结论 HS能显著减轻缺血再灌注大鼠肝细胞线粒体损伤，并能有效抑制线粒体氧化应激。.

Abstract:
Objective To study the effect of hydrogen-rich saline on the prevention of liver mitochondrial injury induced by ischemia-reperfusion (I/R) in rats.

Methods Male Sprague-Dawley rats (n=42) were randomly divided into three experimental groups (n= 14), namely, sham-operated, I/R plus saline-treated (5 mL/kg, i.p.), and I/R plus hydrogen-rich saline-treated (5 mL/kg, i.p.) groups. Mitochondrial oxidative stress and functions, as well as morphologic changes in the mitochondria, were detected 180 min after reperfusion.

Results After I/R, mitochondrial swelling and vague cristae were observed. Mitochondrial functions were significantly decreased, but mitochondrial MDA and GSSH contents were increased by I/R. Hydrogen-rich saline reduced these markers, improved mitochondrial ATP content and respiratory function, and relieved morphological mitochondrial injury (P < 0.01).

Conclusion Hydrogen-rich saline attenuated I/R-induced liver mitochondrial damage and reduced mitochondrial oxidative stress.

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