左铎, 罗艺, 郭华, 张宁. 联合检测血清miR-125b和AFP对原发性肝细胞癌的诊断价值[J]. 中国肿瘤临床, 2014, 41(10): 662-666. DOI: 10.3969/j.issn.1000-8179.20140337
引用本文: 左铎, 罗艺, 郭华, 张宁. 联合检测血清miR-125b和AFP对原发性肝细胞癌的诊断价值[J]. 中国肿瘤临床, 2014, 41(10): 662-666. DOI: 10.3969/j.issn.1000-8179.20140337
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ZUO Duo, LUO Yi, GUO Hua, ZHANG Ning. The diagnosis value of combined detection of serum mir-125b and alpha-fetoprotein for primary hepatocellular carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2014, 41(10): 662-666. DOI: 10.3969/j.issn.1000-8179.20140337
Citation: ZUO Duo, LUO Yi, GUO Hua, ZHANG Ning. The diagnosis value of combined detection of serum mir-125b and alpha-fetoprotein for primary hepatocellular carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2014, 41(10): 662-666. DOI: 10.3969/j.issn.1000-8179.20140337
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联合检测血清miR-125b和AFP对原发性肝细胞癌的诊断价值

The diagnosis value of combined detection of serum mir-125b and alpha-fetoprotein for primary hepatocellular carcinoma

    摘要
  • 摘要:
      目的   探讨血清中miR-125b作为原发性肝细胞癌（hepatocellular carcinoma，HCC）的血清标志物的可能性和联合检测miR-125b和AFP对HCC的诊断价值。
      方法   通过实时荧光定量PCR（quantitative real-time polymerase chain reaction，RT-qPCR）检测65例HCC患者和30例健康对照组的血清miR-125b表达量，分析其对HCC的诊断价值，以及与HCC临床病理资料参数间的关系。
      结果   HCC患者组血清miR-125b表达与对照组相比较低，且两者的差异有统计学意义（P < 0.05）。miR-125b的表达与患者性别、年龄、乙肝表面抗原阳性、AFP、ALT和α-GGT的表达水平无关（P > 0.05）。miR-125b的表达与患者肝硬化、肿瘤大小和TNM分期有关，且差异有统计学意义（P < 0.05）。单独检测miR-125b的ROC曲线下的AUC为0.917（95%CI：0.851~0.960，P < 0.001），灵敏性为85.9%，特异性为93.5%，联合miR-125b和AFP检测的ROC曲线下的AUC为0.951（95%CI：0.894~0.982，P < 0.001），灵敏性为92.9%，特异性为93.5%，检测AFP < 20 μg/L的HCC患者血清miR-125b的ROC曲线下的AUC为0.889（95%CI：0.776~0.957，P < 0.001），灵敏性为84.0%，特异性为87.1%。
      结论   联合检测血清miR-125b和AFP对早期诊断原发性HCC具有重要的临床价值。

     

    Abstract:
      Objective   To investigate the possibility of miR-125b in serum as a novel tumor marker for primary hepatocellular carcinoma (HCC) and the diagnosis value of combined detection of miR-125b and alpha-fetoprotein (AFP) for HCC.
      Methods   We detected serum miR-125b expression of 65 cases of HCC patients and 30 cases of healthy controls by real-time quantitative PCR. Moreover, we analyzed the significance of miR-125b and explored the relationship between miR-125b and clinical pathological factors.
      Results   The level of miRNA-125b was down regulated in serum of HCC patients compared with healthy controls which showed significant differences (P < 0.05). Furthermore, the expression of miRNA-125b has no distinct correlation with sex, age, HbsAg, AFP, ALT and α-GGT, which had no significant differences (P > 0.05). The expression level of miRNA-125b correlated the difference with liver Cirrhosis, tumor size and tumor node metastasis (TNM) stages, which were considered significant differences (P < 0.05). The receiver operating characteristic (ROC) curve analysis of serum miR-125b for the diagnosis of HCC yielded AUC of 0.917(95%CI: 0.851~0.960, P < 0.001)with 85.9% sensitivity and 93.5% specificity. The ROC curve analysis of combined miR-125b and AFP for HCC detection yielded AUC of 0.951(95%CI: 0.894~0.982, P < 0.001)with 92.9% sensitivity and 93.5% specificity. The ROC curve analysis of serum miR-125b as biomarkers for the group (AFP < 20 μg/L) of HCC yielded AUC of 0.889(95%CI: 0.776~0.957, P < 0.001)with 84.0% sensitivity and 87.1% specificity.
      Conclusions   Serum miRNA-125b combined with AFP has considerable clinical value for the early diagnosis of primary HCC

     

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