Abstract: Liposome is an artificially prepared spherical vesicle that has a phospholipid bilayer.Given that the basic structure of its biological membrane is also a lipid bilayer membrane, liposome shares similar structures with body cells Therefore, liposome has good biocompatibility and advantages such as biodegradability, low immunogenicity, and subtle toxicity.Liposome has been widely applied as an effective drug carrier.Studies on liposome-encapsulated fluorescent dye on tumor tracing have been reported in recent years. Liposome can become a more advantageous transport carrier with continuous development of surface modification materials and preparation methods.The long cycle, targeted liposome-encapsulated drugs, and fluorescent dye have become the focus of interest for several researchers.This article mainly discusses the application and progress of long cycle and targeted liposome in cancer research.