Effects of Salviae Miltiorrhizae Liguspyragine Hydrochloride and Glucose Injection on the prevention and treatment of acute radiation-induced pneumonitis in rats
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摘要:
目的 本文利用慢病毒介导的RNA干扰，在结直肠癌裸鼠皮下移植瘤模型中研究信号素4D（Sema4D）对血管新生的影响。 方法 选取体重200±14g成年雄性大鼠105只，分为7组，即正常对照组（N），单独给药组（D），单独照射组（Z），照射10 Gy+给药组（Z10），照射15 Gy+给药组（Z15），照射20 Gy+晚期给药组（照射后4周给药，Z20a），照射20 Gy+早期给药组（照射同时给药，Z20b）。所有药物均经腹腔注射。分别于照射后2、4、6、8、10 W处死大鼠，取血清，应用ELISA法检测TNF-α和TGF-β1，取肺组织制成石蜡切片，HE染色观察病理变化。 结果 单纯照射组血清TNF-α和TGF-β1值明显高于其他各组（P＜0.01），照射加给药组血浆TNF-α和TGF-β1值高于对照组及单独给药组（P>0.05）；各照射加给药组之间TNF-α和TGF-β 1值差异无统计学意义（P>0.05）；大鼠单独照射组，照射2 W后，可见肺脏体积增大，充血水肿，照射后4周，肉眼见肺表面较多点状出血；而给药组肺表面出血点明显减少；参芎组肺组织炎症变化明显好于单独照射组；照射20 Gy大鼠4周后开始给药略好于单独照射组，但差异无统计学意义（P>0.05）；照射4~6周时单纯照射组有大鼠死亡；照射8~10周部分大鼠出现胸腔积液。 结论 参芎注射液能预防放射性肺损伤的发生，机理可能与该药物能降低血浆TNF-α和TGF-β1值有关；发生放射性肺炎后给药能减缓症状，但不能逆转放射性肺炎的病理变化。 Abstract:Objective Semaphorin 4D (Sema4D) acts as a regulator for axon guidance in central nervous system development.However, new evidence indicates that Sema4D has a previously unrecognized function, namely, compensatory angiogenic factor. Thisstudy aimed to investigate the effect of Sema4D on tumor growth and vascularity of colorectal carcinoma (CRC) in nude mice. Methods A total of 70 adult male rats weighing about 200 g were selected and divided into seven groups. These groups were as follows: 1) normal control group (N): rats were injected with 1 ml of normal saline per day; 2) single medicine treatment group (D): rats were administered with 1 ml of Salviae Miltiorrhizae Liguspyragine Hydrochloride and Glucose Injection per day; 3) single irradiation group (Z): rats were exposed to 20 Gy single whole-chest irradiation and injected with 1 ml of normal saline per day; 4) irradiation with 10 Gy and medicine treatment group (Z10): rats were exposed to 10 Gy single whole-chest irradiation and administered with 1 ml of Salviae Miltiorrhizae Liguspyragine Hydrochloride and Glucose Injection per day; 5) irradiation with 15 Gy and medicine treatment group (Z15): rats were exposed to 15 Gy single whole-chest irradiation and administered with 1 ml of Salviae Miltiorrhizae Liguspyragine Hydrochloride and Glucose Injection per day; 6) irradiation with 20 Gy and medicine treatment group (Z20a): rats were exposed to 20 Gy single whole-chest irradiation for four weeks, and administered with 1 ml of Salviae Miltiorrhizae Liguspyragine Hydrochloride and Glucose Injection per day; and 7) irradiation with 20 Gy and medicine treatment group (Z20b): rats were exposed to 20 Gy single whole-chest irradiation and administered with 1 ml of Salviae Miltiorrhizae Liguspyragine Hydrochloride and Glucose Injection per day. Two rats were selected and sacrificed at the end of two, four, six, eight, and ten weeks of irradiation. Samples of blood and lung tissues in rats were obtained. Results In the group with single irradiation, the tumor necrosis factor-α (TNF-α) and transforming growth factor-β (TGF-β) levels in plasma of rats were higher than those in the other groups. In the group with irradiation and medicine treatment, the TNF-α and TGF-β levels in plasma were higher than those in the normal control group and single medicine treatment group. In the group with single irradiation for four weeks some petechial hemorrhages on the surface of the lung were visible to the naked eye. In the groups with medicine treatment, the petechial hemorrhages on the surface of the lung visibly reduced. According to the pathological mechanism of lung tissues, the groups with Salviae Miltiorrhizae Liguspyragine Hydrochloride and Glucose Injection exhibited less inflammation than the single irradiation group. Irradiation at 20 Gy for four weeks followed by a daily abdominal injection was slightly better than single irradiation, but the effects were not obvious. Conclusion Salviae Miltiorrhizae Liguspyragine Hydrochloride and Glucose Injection could prevent the occurrence of lung injury by reducing the TNF-α and TGF-β levels in plasma. After the occurrence of radiation-induced pneumonitis, the application of medicine could not decrease the symptoms. -
图 1 照射后不同时间点大鼠脱毛情况
Figure 1. Rat hair removal at different time points after irradiation
A. Irradiation of 2W; B. Irradiation of 4W; C. Irradiation of 8W; D. Irradiation of 10W
图 2 照射2W肺大体标本
Figure 2. Gross lung specimen at 2W after irradiation
A. The normal control group; B. The single irradiation group; C. The irradiation with 15GY and medicine treatment group; D. The irradiation with 20GY and medicine treatment group
图 3 照射4W肺大体标本
Figure 3. Gross lung specimen at 4Wafter irradiation
A. The normal control group; B. The single irradiation group; C. The irradiation with 15GY and medicine treatment group; D. The irradiation with 20GY and medicine treatment group
图 4 照射4W肺组织病理变化（H & E×400）
Figure 4. The pathological changes of lung tissue at 4W after irradiation(H&E×400)
A. The normal control group; B. The single irradiation group; C. The single medicine treatment group; D. The irradiation with 10GY and medicine treatment group; E. The irradiation with 15GY and medicine treatment group; F. The irradiation with 20GY and medicine treatment group
图 5 照射10W肺组织病理变化（H & E×400）
Figure 5. The pathological changes of lung tissue at 10W after irradiation(H&E×400)
A. The normal control group; B. The single irradiation group; C. The single medicine treatment group; D. The irradiation with 10GY and medicine treatment group; E. The irradiation with 15GY and medicine treatment group; F. The irradiation with 20GY and medicine treatment group
表 1 放射后胸腔积液发生率
Table 1. Incidence of pleural effusion after radiation
表 2 各组TGF-β值, TNF-α值的情况（pg/mL）
Table 2. TGF-β and TNF-α levels of each group (pg/mL)
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