Abstract:
Objective To investigate the expression of microRNA-100 (miR-100) in the serum of gastric cancer patients and its clinical significance.
Methods At The First Affiliated Hospital of Bengbu Medical College, 40 gastric cancer patients who underwent surgery with complete follow-up data and 40 healthy controls were selected as research subjects. Total miRNA was isolated from the se-rum samples of the cancer patients. A stable and sensitive detection method for the absolute quantification of miR-100 was established. The serum levels of miR-100 in the patients and healthy controls were tested according to this novel method. Differences of the miR-100 expression in the serum samples of the patients and healthy controls were analyzed using statistical analysis. The correlation between miR-100 expression levels and the clinicopathological features of gastric cancer was also analyzed.
Results The expression level of miR-100 in the serum was significantly higher in cancer patients(2.78±1.92) fmol/Lthan in the healthy controls(0.19± 0.15) fmol/L, P < 0.01. The receiver operating characteristic curve of miR-100 indicated that serum miR-100 has satisfactory specificity and sensitivity for the diagnosis of gastric cancer (AUC=0.985). Further analytical results indicated that no significant differences were found among the expression levels of serum miR-100 with respect to the gender, age, tumor diameter, infiltrating depth, differentiation degree, frequency of lymph-node metastasis, and TNM stage (P > 0.05).
Conclusion Serum miR-100 testing may be helpful in the diag-nosis of gastric cancer.