王利平, 王启之, 邓敏, 燕善军, 汪建超, 李大鹏, 汪强武. MicroRNA-100在胃癌患者血清中的表达及临床意义[J]. 中国肿瘤临床, 2014, 41(24): 1587-1590. DOI: 10.3969/j.issn.1000-8179.20140777
引用本文: 王利平, 王启之, 邓敏, 燕善军, 汪建超, 李大鹏, 汪强武. MicroRNA-100在胃癌患者血清中的表达及临床意义[J]. 中国肿瘤临床, 2014, 41(24): 1587-1590. DOI: 10.3969/j.issn.1000-8179.20140777
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WANG Liping, WANG Qizhi, DENG Min, YAN Shanjun, WANG Jianchao, LI Dapeng, WANG Qiangwu. Expression and clinical significance of serum microRNA-100 in gastric cancer patients[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2014, 41(24): 1587-1590. DOI: 10.3969/j.issn.1000-8179.20140777
Citation: WANG Liping, WANG Qizhi, DENG Min, YAN Shanjun, WANG Jianchao, LI Dapeng, WANG Qiangwu. Expression and clinical significance of serum microRNA-100 in gastric cancer patients[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2014, 41(24): 1587-1590. DOI: 10.3969/j.issn.1000-8179.20140777
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MicroRNA-100在胃癌患者血清中的表达及临床意义

Expression and clinical significance of serum microRNA-100 in gastric cancer patients

    摘要
  • 摘要:
      目的  探讨MicroRNA-100（miR-100）在胃癌患者血清中的表达及临床意义。
      方法  选择蚌埠医学院第一附属医院手术及随访资料完整的胃癌患者（40例）和健康对照者（40例）为研究对象，提取血清总miRNA，在建立了稳定、敏感的血清miR-100绝对定量检测方法（qRT-PCR）的基础上，检测胃癌和健康对照者血清中miR-100表达水平。分析胃癌和健康对照者血清中miR-100表达差异及血清中miR-100表达水平与胃癌临床病理参数的关系。
      结果  胃癌患者血清中miR-100的表达水平为（2.78±1.92）fmol/L，显著高于健康对照者（0.19±0.15）fmol/L，P < 0.01，同时miR-100的受试者工作特征曲线显示，血清miR-100对胃癌诊断具有良好的特异度和敏感度（曲线下面积0.985）；进一步分析发现：血清中miR-100的表达与性别、年龄、肿瘤直径大小、浸润深度、分化程度、淋巴结转移数量、TNM分期等无明显差异（P > 0.05）。
      结论  血清miR-100的检测可能有助于胃癌的诊断。

     

    Abstract:
      Objective  To investigate the expression of microRNA-100 (miR-100) in the serum of gastric cancer patients and its clinical significance.
      Methods  At The First Affiliated Hospital of Bengbu Medical College, 40 gastric cancer patients who underwent surgery with complete follow-up data and 40 healthy controls were selected as research subjects. Total miRNA was isolated from the se-rum samples of the cancer patients. A stable and sensitive detection method for the absolute quantification of miR-100 was established. The serum levels of miR-100 in the patients and healthy controls were tested according to this novel method. Differences of the miR-100 expression in the serum samples of the patients and healthy controls were analyzed using statistical analysis. The correlation between miR-100 expression levels and the clinicopathological features of gastric cancer was also analyzed.
      Results  The expression level of miR-100 in the serum was significantly higher in cancer patients(2.78±1.92) fmol/Lthan in the healthy controls(0.19± 0.15) fmol/L, P < 0.01. The receiver operating characteristic curve of miR-100 indicated that serum miR-100 has satisfactory specificity and sensitivity for the diagnosis of gastric cancer (AUC=0.985). Further analytical results indicated that no significant differences were found among the expression levels of serum miR-100 with respect to the gender, age, tumor diameter, infiltrating depth, differentiation degree, frequency of lymph-node metastasis, and TNM stage (P > 0.05).
      Conclusion  Serum miR-100 testing may be helpful in the diag-nosis of gastric cancer.

     

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