倪晓辰, 赵志红, 马永良, 任宗涛, 刘 彬, 樊 博, 卫书飞, 张爱莉. 桧木醇诱导人肾透明细胞癌786-O细胞凋亡及其机制研究*[J]. 中国肿瘤临床, 2015, 42(1): 43-46. DOI: 10.3969/j.issn.1000-8179.20141412
引用本文: 倪晓辰, 赵志红, 马永良, 任宗涛, 刘 彬, 樊 博, 卫书飞, 张爱莉. 桧木醇诱导人肾透明细胞癌786-O细胞凋亡及其机制研究*[J]. 中国肿瘤临床, 2015, 42(1): 43-46. DOI: 10.3969/j.issn.1000-8179.20141412
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Xiaochen NI, Zhihong ZHAO, Yongliang MA, Zongtao REN, Bin LIU, Bo FAN, Shufei WEI, Aili ZHANG. Hinokitiol induces clear cell renal cancer 786-O cell apoptosis via autophagy induction[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2015, 42(1): 43-46. DOI: 10.3969/j.issn.1000-8179.20141412
Citation: Xiaochen NI, Zhihong ZHAO, Yongliang MA, Zongtao REN, Bin LIU, Bo FAN, Shufei WEI, Aili ZHANG. Hinokitiol induces clear cell renal cancer 786-O cell apoptosis via autophagy induction[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2015, 42(1): 43-46. DOI: 10.3969/j.issn.1000-8179.20141412
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桧木醇诱导人肾透明细胞癌786-O细胞凋亡及其机制研究*

Hinokitiol induces clear cell renal cancer 786-O cell apoptosis via autophagy induction

    摘要
  • 摘要: 目的：探讨桧木醇（hinokitiol ）对人肾透明细胞癌786-O细胞株的增殖抑制作用及诱导凋亡效应，初步阐明其机制。方法：采用CCK-8 法检测桧木醇对肾癌786-O细胞增殖的抑制作用；流式细胞术检测细胞凋亡情况；转染EGFP-LC3 后镜下观察细胞自噬状态；采用Western blot对细胞Caspase- 3 剪切体、LC3 和P62蛋白表达量进行检测。结果：桧木醇可以显著抑制肾癌786-O细胞增殖，通过激活Caspase途径诱导细胞凋亡。桧木醇诱导肾癌786-O细胞发生自噬，LC3 蛋白表达显著上调，而P62蛋白表达下调。结论：桧木醇可显著抑制肾癌786-O细胞的增殖并通过过度激活细胞自噬促进肾癌细胞凋亡。

     

    Abstract: Objective: To investigate the effects of hinokitiol on the proliferative inhibition and apoptosis induction in human clear cell renal cancer 786-O cells. Methods:CCK-8 assays were performed to analyze the effects of hinokitiol on the proliferation of 786-O cells. The apoptosis rate was determined by flow cytometry. EGFP-LC 3 microscopy assays were performed to assess the autoph-agy flux. Cleaved Caspase-3, LC 3, and P 62were detected by Western blot. Results: Hinokitiol could inhibit the proliferation of the 786-O cells and could induce cell apoptosis via Caspase pathway. Hinokitiol induced the autophagy of 786-O cells, increased LC3 ex  pression, and downregulated P 62expression. Conclusion:Hinokitiol can inhibit the proliferation of 786-O cells and can induce cell apoptosis via autophagy induction.

     

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