齐丽莎①, 刘志勇①, 宋汪昭①, 程润芬①, 孙保存①②. Dickkopf-1 对结肠癌组织血管生成拟态形成的抑制作用及相关机制研究*[J]. 中国肿瘤临床, 2015, 42(20): 1007-1011. DOI: 10.3969/j.issn.1000-8179.2015.20.179
引用本文: 齐丽莎①, 刘志勇①, 宋汪昭①, 程润芬①, 孙保存①②. Dickkopf-1 对结肠癌组织血管生成拟态形成的抑制作用及相关机制研究*[J]. 中国肿瘤临床, 2015, 42(20): 1007-1011. DOI: 10.3969/j.issn.1000-8179.2015.20.179
shu
Lisha QI1, Zhiyong LIU1, Wangzhao SONG1, Runfen CHENG1, Baocun SUN1. Dickkopf-1 inhibits vasculogenic mimicry formation of colon cancer and relevant mechanism[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2015, 42(20): 1007-1011. DOI: 10.3969/j.issn.1000-8179.2015.20.179
Citation: Lisha QI1, Zhiyong LIU1, Wangzhao SONG1, Runfen CHENG1, Baocun SUN1. Dickkopf-1 inhibits vasculogenic mimicry formation of colon cancer and relevant mechanism[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2015, 42(20): 1007-1011. DOI: 10.3969/j.issn.1000-8179.2015.20.179
shu

Dickkopf-1 对结肠癌组织血管生成拟态形成的抑制作用及相关机制研究*

Dickkopf-1 inhibits vasculogenic mimicry formation of colon cancer and relevant mechanism

    摘要
  • 摘要: 目的：探讨结肠癌组织中Dickkopf-1（Dkk 1）表达对血管生成拟态（vasculogenic mimicry ，VM）的抑制作用及相关机制。方法：收集2002年1 月至2004年12月间天津医科大学肿瘤医院收治的217 例结肠癌患者资料，利用CD34-PAS对结肠癌组织进行双重染色及免疫组化方法检测并分析VM与Dkk 1 表达的关系；利用体外三维培养（three dimensional culture，3Dculture）观察Dkk 1 对人结肠癌HCT 116 细胞管状结构形成能力及血管内皮钙黏蛋白（VE-cadherin）表达的影响；建立裸鼠皮下移植瘤模型进一步明确Dkk 1 对肿瘤组织内VM形成的抑制作用。结果：存在VM的结肠癌组织中Dkk 1 表达较低（P < 0.05）；Dkk 1 过表达的HCT 116 细胞形成管状结构能力明显减弱，且VE-cadherin表达降低；Dkk 1 可抑制裸鼠移植瘤组织中HCT 116 细胞形成VM。结论：Dkk 1 过表达可抑制结肠癌中VM形成。

     

    Abstract: Objective: To investigate the inhibitory effect of Dickkopf- 1 (Dkk1) on vasculogenic mimicry (VM) formation and the relevant mechanism. Methods:CD34-PAS dual staining and immunohistochemical staining were used to detect and analyze the re -lationship between VM existence and Dkk 1 expression in 217 human coloncancer tissue samples; three dimensional (3D) culture was used to detect the influence of Dkk1 on tube structure formation and on VE-cadherin expression; a subcutaneous mouse xenograft mod -el was made to further validate the inhibitory role of Dkk 1 on VM formationin vivo . Results: VM-positive samples indicated a lower expression of Dkk1(P<0.05); colon cancer cells with Dkk1 overexpression exhibited a decreased ability to form tube-like structure and a decreased expression of VE-cadherin; Dkk 1 inhibited the VM-formation abilities of human colorectal carcinoma cell line xenograft tu -mor tissue.Conclusion:Dkk1 inhibits the VM formation of colon cancer.

     

    • HTML全文
    • 参考文献(0)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回