Abstract: Objective:To identify the signature of tumor-infiltrating lymphocyte (TIL) subtypes that may affect cytokine expres -sion between different outcomes of hepatocellular carcinoma (HCC) patients by analyzing the CD molecular expression profiles of non -cancerous hepatic tissues. Methods:Surface markers of TIL in noncancerous hepatic tissues from 146 HCC patients were determined by using immunohistochemical method and flow cytometry. Univariate and multivariate Cox proportional hazards models and Kaplan-Meier method were used to analyze the association of their expression levels with tumor recurrence and survival.Results: More than 86.4% of TILs in patients were quiescent, as measured via CD4 + or Foxp3 expression. Meanwhile, more than 90% of CD3 +T cells ex-pressed CD 8 +. The proportion of T cells was low compared with CD 8 + T cells. The proportion of CD 19and CD20in distant nontumor tissues almost was zero. The proportion of T cell subgroups isolated from HCC circulating whole blood did not show a significant shift compared with the normal control, as follows: CD4 +T/CD 8 +T =1.167 ± 1.04, CD 8 + T/CD3 +T =0.288 ± 0.116, and CD 4 +T/CD 3 +T =0.429 ± 0.178. The proportion of CD 8 + T cells in noncancerous hepatic tissues was higher than that in blood ( P<0.001). Conclusion: TILs in HCC noncancerous hepatic tissues are increased and contain a subpopulation of CD3 +CD 8 +T cells. CD8 +T cells in cancerous tissues, rather than noncancerous tissues, show significant differences between different prognostic groups.