Abstract: Objective:To analyze the expression of MUC 15and PI 3K/Akt in gastric carcinoma and its association with clinicopathologi-cal characteristics and prognosis. Methods:The expression of MUC 15and Akt was detected in 144 cases of gastric carcinoma tissues and corresponding para- carcinoma tissues by tissue microarray and immunohistochemistry.Results: The positive expression rate of MUC15in gastric carcinoma was 79. 8%, higher than that of para-carcinoma tissues (22. 2%, P<0. 01). The positive expression rate of Akt protein in gastric carcinoma was80. 6%, higher than that of para-carcinoma tissues (16. 7%, P<0. 01). The expression of MUC15and Akt was statistically associated with the grades of differentiation, invasion depth, lymphatic metastasis, TNM stage of tumor tissues ( P<0. 05), and the positive correlation between the two protein expression that appear in the gastric tumor tissue ( P=0. 001 ). Univariate survival analysis showed that the over-expression of either MUC 15or Akt was inversely correlated with the survival time (P<0. 05and P<0. 01, respectively). Cox multiple regression analysis indicated that patients with over- expression of both MUC 15and Akt had the worst prognoses (HR=3. 115 , P<0. 05). Conclusion: MUC15may be involved in the occurrence, development, invasion, and metastasis of gastric cancer through the PI 3K/Akt cell signaling pathway, and the expression of MUC 15combined with Akt is a powerful predictor for the prognosis of gastric cancer.