杨康, 汤小东, 郭卫. LDN-193189对人去分化软骨肉瘤细胞系NDCS-1 的抑制作用研究*[J]. 中国肿瘤临床, 2016, 43(2): 52-55. DOI: 10.3969/j.issn.1000-8179.2016.02.293
引用本文: 杨康, 汤小东, 郭卫. LDN-193189对人去分化软骨肉瘤细胞系NDCS-1 的抑制作用研究*[J]. 中国肿瘤临床, 2016, 43(2): 52-55. DOI: 10.3969/j.issn.1000-8179.2016.02.293
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Kang YANG, Xiaodong TANG, Wei GUO. LDN-193189inhibits progression and induces apoptosis in human dedifferentiated chondrosarcoma cell line NDCS-1[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2016, 43(2): 52-55. DOI: 10.3969/j.issn.1000-8179.2016.02.293
Citation: Kang YANG, Xiaodong TANG, Wei GUO. LDN-193189inhibits progression and induces apoptosis in human dedifferentiated chondrosarcoma cell line NDCS-1[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2016, 43(2): 52-55. DOI: 10.3969/j.issn.1000-8179.2016.02.293
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LDN-193189对人去分化软骨肉瘤细胞系NDCS-1 的抑制作用研究*

LDN-193189inhibits progression and induces apoptosis in human dedifferentiated chondrosarcoma cell line NDCS-1

    摘要
  • 摘要: 目的：检测骨形成蛋白（BMP ）受体抑制剂LDN-193189对人去分化软骨肉瘤（DDCS）细胞系NDCS- 1 的抑制作用，探讨LDN-193189对去分化软骨肉瘤的抑癌机制。方法：以5 nmol/L 的LDN-193189作用于NDCS- 1 细胞，MTT 、平板克隆法检测LDN-193189对NDCS- 1 细胞的增殖抑制作用，Transwell 法、划痕实验检测LDN193189对NDCS- 1 细胞的侵袭抑制作用，Westernblot检测BMPR 2、p-Smad1/ 5 及RUNX 2 的蛋白表达抑制情况。结果：药物处理后NDCS- 1 细胞增殖、侵袭被明显抑制；药物处理后NDCS- 1 细胞的BMPR 2、p-Smad1/ 5 及RUNX 2 蛋白表达下降。结论：LDN-193189通过抑制BMPR 2-p-Smad 1/ 5-RUNX2 信号传导通路能有效抑制去分化软骨肉瘤细胞系NDCS- 1 的增殖侵袭能力。

     

    Abstract: Objective:To clarify the effects of the BMP receptor inhibitor LDN-193189 in the dedifferentiated chondrosarcoma (DDCS) cell line NDCS-1 and to explore the anti-tumor mechanism of LDN- 193189 in DDCS. Methods: NDCS-1 was treated with 5 nmol/L of LDN- 193189 . MTT assay and clone formation experiments were used to verify that LDN- 193189 suppressed cell proliferation. Transwell and wound healing tests were performed to demonstrate that LDN-193189 inhibited cell invasion. Western blot detection was used to show that LDN- 193189 inhibited the suppression of BMPR 2, p-Smad 1/5, and RUNX 2 protein expression. Results:The BMPR2 signaling pathway was inhibited by LDN- 193189 ; thus, cell viability and invasion were significantly suppressed. Conclusion: LDN- 193189 induces the inhibition of progression in vitro via the BMPR2-p-Smad 1/5-RUNX 2 signaling pathway in the human DDCS cell line NDCS- 1.

     

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