Abstract: Objective:To investigate pathological characteristics and prognosis of patients with Epstein-Barr virus (EBV) positive gastric diffuse large B-cell lymphoma (DLBCL).Methods:Through retrospective study, we collected 75cases of patients with DLBCL that oc-curs in the stomach. The patients were divided into two groups consisting of 60cases of EBV negative control group and 15cases of EBV positive group. To analyze the pathological characteristics and prognosis of patients with EBV positive gastric DLBCL, immunohisto -chemical and Epstein-Barr encoding region (EBER) in situ hybridization methods were used to detect Bcl- 2, c-myc protein expression, and EBV-encoded RNA (EBER). Results:In certain aspects of clinical manifestations, such as age, gender, and origin, the comparison be  tween EBV-positive and EBV-negative groups had no statistically significant difference. The same results were obtained for Bcl- 2 and c-myc protein expression. However, a statistically significant difference (P=0. 01) was observed under the R-CHOP regimen where the me -dian overall survival (OS) of the EBV- positive and EBV- negative groups were 15. 1 and 31. 4 months, respectively. Conclusion: In pa-tients with DLBCL of the stomach, the EBV infection had no obvious effects in terms of clinical manifestation, origin, morphology, and protein expression of tumor cells. EBV-positive DLBCL was not limited to elderly patients. Under the R-CHOP regimen, the prognosis of EBV-positive patients was worse than that of EBV-negative patients.