孙健, 王晓波, 张旗, 慕俐君. EZH2 在急性髓系白血病病程中的表达和对预后判断的意义研究[J]. 中国肿瘤临床, 2016, 43(16): 727-730. DOI: 10.3969/j.issn.1000-8179.2016.16.518
引用本文: 孙健, 王晓波, 张旗, 慕俐君. EZH2 在急性髓系白血病病程中的表达和对预后判断的意义研究[J]. 中国肿瘤临床, 2016, 43(16): 727-730. DOI: 10.3969/j.issn.1000-8179.2016.16.518
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Jian SUN, Xiaobo WANG, Qi ZHANG, Lijun MU. Research progress and prognostic significance of EZH2 in acute myeloid leukemia[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2016, 43(16): 727-730. DOI: 10.3969/j.issn.1000-8179.2016.16.518
Citation: Jian SUN, Xiaobo WANG, Qi ZHANG, Lijun MU. Research progress and prognostic significance of EZH2 in acute myeloid leukemia[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2016, 43(16): 727-730. DOI: 10.3969/j.issn.1000-8179.2016.16.518
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EZH2 在急性髓系白血病病程中的表达和对预后判断的意义研究

Research progress and prognostic significance of EZH2 in acute myeloid leukemia

    摘要
  • 摘要: 目的：探讨DNA 甲基转移酶（DNAmethyltransferase，DNMT 1）与EZH 2 在急性髓系白血病（acutemyeloidleukemia，AML ）中的表达及其相关性。方法：采用荧光定量PCR（fluorescencequantitativePCR ，FQ-PCR）检测大连医科大学附属第二医院2014年1月至2015年1 月50例初治AML 患者骨髓细胞中DNMT 1 与EZH 2 mRNA 的表达水平，分析DNMT 1 表达水平与临床特征和预后的关系以及与EZH 2 的相关性。结果：50例AML 患者骨髓细胞中DNMT 1 mRNA 的表达显著高于30例正常供者（2.72± 0.73vs . 0.89±0.27，P < 0.01）；EZH 2 的表达水平也显著高于正常供者（4.39± 1.06vs . 1.87± 0.33，P < 0.01）；EZH 2 与DNMT 1 的表达水平呈显著正相关（r = 0.51，P = 0.002）；DNMT 1 表达水平与外周血幼稚细胞比例≥ 60%（P < 0.05）、WBC≥ 50× 109/L （P < 0.05）呈显著相关；DNMT 1 高表达组患者中位生存时间15个月（95%CI 为9～19个月），显著低于DNMT 1 低表达组患者的32个月（95%CI 为27～40个月，P =0.006）。结论：DNMT 1 和EZH 2 在AML 患者中均高表达，两者表达水平呈正相关，DNMT 1 与AML 患者预后不良相关。

     

    Abstract: Objective:To investigate the relationship between DNA methyltransferase (DNMT1) and EZH2 gene expression levels and their clinical significance in patients with acute myeloid leukemia (AML). Methods:The mRNA expression levels of DNMT1 and EZH2 in 50AML cases and30healthy controls were quantified through real-time PCR. The relationship among DNAmethyltransferase(DNMT1) and EZH2, clinicopathological factors, and prognosis was analyzed. Results:The mRNA expression of DNMT 1 in the AML cases ( 2. 72±0. 73) was significantly higher than that in the healthy controls ( 0. 89± 0. 27) ( P<0. 01). The mRNA expression of EZH 2 in the AML cases (4. 39± 1. 06) was also significantly higher than that in the healthy controls ( 1. 87± 0. 33) ( P<0. 01). The expression of DNMT 1 was positively associated with that of EZH 2 ( r=0. 51, P=0. 002 ). The expression of DNMT1 was also associated with PB% and WBC ≥ 50× 109/L ( P<0. 05). The overall survival of the group with a high-mRNA-expressing DNMT 1 was 15months (95% CI=9- 19months). This period was significantly shorter than that of the group with low-mRNA-expressing DNMT 1 (32months,95% CI=27- 40months;P=0. 006 ). Conclu -sion: DNMT1 and EZH2 expression levels were downregulated. These levels were associated with poor AML prognosis. The expression of DNMT 1 was also positively associated with that of EZH 2.

     

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