Abstract: Objective:To determine the effect of hypoxic stress on glioma cell XBP1 expression, the relationship between XBP 1 expres -sion and sugar metabolism, the influence of XBP 1 repression on the survival rate of glioma cells under normoxia and hypoxia, and the influence of XBP 1 on glioma cell glycolysis.Methods:We tested XBP1 activation in human glioma cell lines cultured under normoxia and hypoxia. XBP1 expression was repressed with siRNA technology. Cells were treated with oxidative phosphorylation inhibitor. We then detected the variation in cell apoptosis, sugar metabolism mode, and cell apoptosis and glycolysis products under normoxia and hypoxia. Results:XBP 1 activation increased under hypoxia. Silencing XBP1 expression reduced glioma cell survival level, ATP and lactic acid production, and glucose consumption under hypoxia. After inhibiting cell oxidative phosphorylation, XBP 1 repression significantly reduced the survival level of glioma cells. Conclusion: Hypoxia can activate XBP1 in glioma cells. Under hypoxia, XBP 1 silencing de -presses cell activity and glycolysis. Glycolysis of glioma cells under hypoxia depends on XBP 1 activation.