Abstract:
Objective To explore the correlation between single nucleotide polymorphisms (SNPs) in Homo sapiens longevity assurance homologue 2 (LASS2) gene 3′-untranslated regions (UTR) and susceptibility of bladder cancer among residents of Yunnan, China.
Methods A total of 105 bladder cancer patients (bladder cancer group) and 100 nonbladder cancer patients (control group) were selected. PCR method and sequence for LASS2-3′-UTR were performed to identify the SNPs correlated with bladder cancer. The relationships between the LASS2-3′- UTR polymorphisms and bladder cancer risk were analyzed.
Results An SNP (rs8444) was identified in LASS2-3′-UTR, and the T/C allele frequencies and genotype distributions of rs8444 largely differed between the bladder cancer and control groups (χ2=10.267, P=0.006; χ2=10.634, P=0.001). Individuals that carry the rs8444 C allele or CC genotype had a remarkably lower risk of bladder cancer compared with those that carry the T allele or TT genotype (OR=0.489, 95%CI: 0.309-0.772, P=0.002; OR= 0.258, 95%CI: 0.081-0.827, P=0.023). No significant correlations were observed between the T/C allele frequencies and genotype distributions of rs8444 and TNM stage, as well as histological grade and distant metastasis in bladder cancer (P>0.05).
Conclusion The rs8444 C allele or CC genotype located within LASS2-3′-UTR can lower the susceptibility of bladder cancer among the residents of Yunnan, China. However, it is not associated with the TNM stage, histological grade, and distant metastasis.