Abstract:
Objective To investigate the effect and mechanism of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in modulating the growth and invasion of oral squamous cell carcinoma (OSCC).
Methods MALAT1 siRNA was transfected into SCC25 and UM1 human OSCC cell lines. Cell counting kit-8 assay and flow cytometry were used to test the proliferation, cell cycle, and apoptosis of the cells after infection by the MALAT1 siRNA. Cell invasive and migration ability were evaluated by Transwell assay and cell migration assay, respectively. The expression levels of the proteins VHL and β-catenin, which regulate the cell cycle, apoptosis, epithelialmesenchymal transition, invasion, and migration, were examined by Western blot assay.
Results After down regulation of the MALAT1 expression in the cells, the proliferation was inhibited, and G1/S arrest was triggered. The expression of cyclin D1 was down regulated and that of P21 was up regulated. Cell apoptosis increased, and the expression levels of Bax and cleaved caspase-3 were up regulated. Migration and invasion were attenuated. The expression levels of N-cadherin, vimentin, and MMP-2/9 were down regulated, and the expression of E-cadherin was up regulated in the cells after the knockdown of MALAT1. These findings show significant differences between the transfected cells and negative-control cells. We found that VHL expression was up regulated and that of β-catenin was down regulated.
Conclusion MALAT1 is an important factor for the growth and invasion of OSCC. MALAT1 possibly modulates these processes through the VHL/β-catenin signal pathway.
下载: