蛋白激酶PLK4对人脑胶质瘤增殖的影响

张作鑫; 周俊虎; 韩磊

doi:10.3969/j.issn.1000-8179.2017.14.300

蛋白激酶PLK4对人脑胶质瘤增殖的影响

Effect of kinase PLK4 on the proliferation of glioma cells in vitro

摘要

摘要:
目的分析蛋白激酶PLK4表达与胶质母细胞瘤患者预后相关性以及对人胶质瘤细胞增殖活性的影响。
方法基于CGGA数据库评估PLK4表达与胶质瘤患者病理级别和预后的关系；构建靶向PLK4基因的小分子干扰RNA，运用qPCR和Western blot法检测转染效率；利用MTT和平板克隆形成实验评价PLK4对肿瘤细胞增殖活性的影响。
结果 PLK4的表达水平随样本病理级别的上升而增加，并且与胶质瘤患者总生存期和高级别胶质瘤患者的预后紧密相关；MTT和平板克隆形成实验结果显示敲低PLK4表达后肿瘤细胞增殖能力明显减弱。
结论 PLK4的表达与胶质瘤患者的预后紧密相关，并能够影响肿瘤细胞的增殖活性。因此，PLK4可能是胶质瘤治疗的潜在靶点。

Abstract:
Objective To analyze the correlation of protein kinase PLK4 with the prognosis of patients with malignant glioma and the proliferation of human glioblastoma cells.
Methods The relationship of PLK4 expression to the pathological grade and prognosis was evaluated using CGGA datasets. Designated siRNAs targeting PLK4 were constructed, and the silencing effect was identified by RT-qPCR and Western blot analyses. The effect of PLK4 on the proliferation of tumor cells was evaluated by MTT and colony formation assays.
Results The expression of PLK4 increased with pathological grade and was significantly related to the overall survival in patients with glioma and the prognosis of patients with high-grade glioma. MTT and colony formation assay results indicated that the proliferation activity was significantly reduced after depleting PLK4.
Conclusion The expression of PLK4 is related to the prognosis of patients with glioma and can affect the proliferation activity of tumor cells. Therefore, PLK4 could be a potential therapeutic target in glioma treatment.

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