Abstract:
Objective To discuss the diagnostic value of miR-26a/b and relationship of this microRNA with clinicopathological features in patients with gastric cancer.
Methods The expression of serum miR-26a/b was detected in 121 patients with gastric cancer and 116 healthy controls using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR).The relationship of miR-26a/b and clinicopathological parameters of patients were analyzed.The receiver operating curve (ROC) was constructed to in vestigate the value of miR-26a/b in the diagnosis of gastric cancer.
Results The relative expression levels of serum miR-26a and miR-26b in patients with gastric cancer were lower than those in the control group (1.60±1.02 vs.5.35±0.44; 1.44±0.71 vs.5.35±0.71; P < 0.05).The relative expression level of miR-26a/b correlated with TNM stage, and the relative expression level of miR-26a also correlated with invasion depth and lymph node metastasis (P < 0.05), but not with sex, age, tumor size, or histological type (P>0.05).The area under the ROC curve of miR-26a was 0.828 (95% CI:0.776~0.881), with sensitivity of 73.3% and specificity of 81.0%, while the area under the ROC curve of miR-26b was 0.853 (95% CI:0.801~0.906), with sensitivity of 68.1% and specificity of 99.2%.
Conclusion The expression of miR-26a/b significantly reduced in patients with gastric cancer, and its expression level correlated with the clinical stage, invasion depth, and lymph node metastasis.MiR-26a/b has potential diagnostic value for gastric cancer.
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