幽门螺杆菌感染与胃癌中PRC2和H3K27me3的表达关系

张宁; 曾智; 阎丽萍; 杨珂; 孙庆文; 黄鹏

doi:10.3969/j.issn.1000-8179.2018.13.212

幽门螺杆菌感染与胃癌中PRC2和H3K27me3的表达关系

doi: 10.3969/j.issn.1000-8179.2018.13.212

张宁^①,,
曾智^②,
阎丽萍^③,
杨珂^④,
孙庆文^⑤,
黄鹏^⑥, ,

①.
上海健康医学院基础医学院（上海市201318）
②.
湖北省人民医院病理科
③.
青岛大学附属中心医院检验科
④.
复旦大学附属上海市第五人民医院病理科
⑤.
复旦大学生命科学院
⑥.
上海健康医学院临床医学院

基金项目:

国家自然科学基金项目 81602535

上海市卫生计生委科研项目 201740161

上海市自然科学基金项目 15ZR1421800

详细信息

作者简介:
张宁专业方向为消化道肿瘤发病机制和临床病理的研究。E-mail：zhangn@sumhs.edu.cn

通讯作者:
黄鹏 huangp_15@sumhs.edu.cn

计量
- 文章访问数: 117
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- 被引次数: 0
出版历程
- 收稿日期: 2018-03-05
- 修回日期: 2018-06-13
- 刊出日期: 2018-07-15

Relationship between Helicobacter pylori infection and the expression of PRC2 and H3K27me3 in gastric cancer

Zhang Ning^{①
,},
Zeng Zhi^②,
Yan Liping^③,
Yang Ke^④,
Sun Qingwen^⑤,
Huang Peng^{⑥
, ,}

①.
School of Basic Medicine, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China
②.
Department of Pathology, People's Hospital of Hubei Province, Hubei 430060, China
③.
Clinical Laboratory, Affiliated Central Hospital of Qingdao University, Qingdao 266042, China
④.
Department of Pathology, the Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, China
⑤.
School of Life Sciences, Fudan University, Shanghai 200438, China
⑥.
School of Clinical Medicine, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China

Funds:

National Natural Science Foundation of China 81602535

Shanghai Municipal Commission of Health and Family Planning 201740161

Science and Technology Commission of Shanghai Municipality 15ZR1421800

More Information

Corresponding author: Peng Huang; huangp_15@sumhs.edu.cn

摘要

摘要: 目的研究幽门螺杆菌（Helicobacter pylori，HP）感染对胃癌组织中多梳抑制复合物2（polycomb repressive complex 2，PRC2）和组蛋白H3K27me3表达的影响，探索HP感染在胃癌发生过程中的作用。方法利用快速尿素酶检测、Giemsa染色和PCR方法检测复旦大学附属上海市第五人民医院2014年1月至2017年10月行胃癌手术的84例患者HP感染情况，应用免疫组织化学法检测胃癌和癌旁组织PRC2和H3K27me3蛋白的表达，分析两者表达与HP感染之间的相关性。结果 3种方法检测HP感染率分别为70.24%、61.90%和76.19%。PRC2家族成员果蝇zeste基因增强子同源物2（enhancer of zeste homolog 2，EZH2）、SUZ12、EED和H3K27me3蛋白在胃癌组织中的阳性表达率分别为79.76%（67/84）、77.38%（65/84）、40.48%（34/84）和69.05%（58/84），均显著高于癌旁组织（22.62%、34.52%、8.33%和14.29%，P < 0.05）。临床资料分析表明，EZH2和H3K27me3蛋白在胃癌中的表达与淋巴结转移呈正相关。在HP阳性胃癌组织中，EZH2、SUZ12、EED和H3K27me3蛋白阳性表达率分别为89.06%（57/64）、84.38%（54/64）、46.88%（30/64）和82.81%（53/64），均显著高于HP阴性胃癌组织（50.00%、55.00%、20.00%和25.00%，P < 0.05）。结论 HP感染与胃癌组织中PRC2和H3K27me3蛋白表达上调相关。
- 幽门螺杆菌 /
- 多梳抑制复合物2 /
- 果蝇zeste基因增强子同源物2 /
- H3K27me3 /
- 胃癌
Abstract: Objective To investigate the effects of Helicobacter pylori (HP) infection on polycomb repressive complex 2 (PRC2) and H3K27me3 expression and to explore the role of HP infection in the development of gastric cancer. Methods Fast urease tests, Giemsa staining, and polymerase chain reaction (PCR) assays were conducted to detect HP in specimens from 84 gastric cancer patients, who had received surgeries in the Fifth People's Hospital of Shanghai from January 2014 to October 2017. Immunohistochemistry staining was applied to examine PRC2 and H3K27me3 expression in gastric cancer tissues and para-tumor tissues. The relationship between HP infection and PRC2 and H3K27me3 expression was analyzed. Results The infection rates of HP were 70.24%, 61.90%, and 76.19% as detected by the three methods, respectively. The expression of enhancer of zeste homolog 2 (EZH2), suppressor of zeste 12 (SUZ12), embryonic ectoderm development (EED), and H3K27me3 protein were detected in 79.76% (67/84), 77.38% (65/84), 40.48% (34/84), and 69.05% (58/84) of gastric cancer tissues, respectively, all of which were significantly higher than those of para-tumor tissues (22.62%, 34.52%, 8.33%, and 14.29%, respectively; P < 0.05). The expression of EZH2 and H3K27me3 were positively correlated with lymph node metastasis, and the expression of these two proteins were also positively correlated. In gastric cancer tissues with HP infection, the expression of EZH2, SUZ12, EED, and H3K27me3 protein were detected at rates of 89.06% (57/64), 84.38% (54/64), 46.88% (30/64), and 82.81% (53/64), respectively, which were significantly higher than in gastric cancer tissues without HP infection (50.00%, 55.00%, 20.00%, and 25.00%, respectively; P < 0.05). Conclusions HP infection is associated with increased expression of PRC2 and H3K27me3 proteins in gastric cancer.
- Helicobacter pylori /
- polycomb repressive complex 2 /
- enhancer of zeste homolog 2 /
- H3K27me3 /
- gastric cancer

HTML全文

图 1 HP检测结果

A：胃癌组织Giemsa染色（Giemsa×40）；B：VacA基因扩增电泳结果，M：DNA marker；1~5泳道：VacA基因扩增产物电泳结果

下载: 全尺寸图片幻灯片

图 2 PRC2家族蛋白和H3K27me3在胃癌组织中的表达（SP法×40）

A：EZH2；B：SUZ12；C：EED；D：H3K27me3

下载: 全尺寸图片幻灯片

表 1 PRC2家族蛋白在胃癌组织及癌旁组织中的表达

表 2 EZH2和H3H27me3蛋白表达与胃癌临床病理特征的关系

表 3 PRC2和H3K27me3蛋白在HP阳性及阴性胃癌组织中的表达

表 4 胃癌组织中EZH2和H3K27me3蛋白表达的关系

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