金荣, 何新阳, 刘成业, 陈志强, 周杭城, 王晓秋. SPNS2在结直肠癌组织中的表达及其临床意义[J]. 中国肿瘤临床, 2018, 45(21): 1091-1095. DOI: 10.3969/j.issn.1000-8179.2018.21.078
引用本文: 金荣, 何新阳, 刘成业, 陈志强, 周杭城, 王晓秋. SPNS2在结直肠癌组织中的表达及其临床意义[J]. 中国肿瘤临床, 2018, 45(21): 1091-1095. DOI: 10.3969/j.issn.1000-8179.2018.21.078
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Jin Rong, He Xinyang, Liu Chengye, Chen Zhiqiang, Zhou Hangcheng, Wang Xiaoqiu. Expression of SPNS2 in colorectal cancer tissues and its clinical significance[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2018, 45(21): 1091-1095. DOI: 10.3969/j.issn.1000-8179.2018.21.078
Citation: Jin Rong, He Xinyang, Liu Chengye, Chen Zhiqiang, Zhou Hangcheng, Wang Xiaoqiu. Expression of SPNS2 in colorectal cancer tissues and its clinical significance[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2018, 45(21): 1091-1095. DOI: 10.3969/j.issn.1000-8179.2018.21.078
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SPNS2在结直肠癌组织中的表达及其临床意义

Expression of SPNS2 in colorectal cancer tissues and its clinical significance

    摘要
  • 摘要:
      目的  探讨鞘氨醇-1-磷酸转运体2（spinster homolog 2，SPNS2）在结直肠癌组织中的表达及其临床意义。
      方法  选取2010年1月至2013年6月于安徽省立医院经结直肠癌手术切除的133例患者的结直肠癌和癌旁正常组织标本，采用免疫组织化学法检测SPNS2蛋白的表达。另收集2018年2月至2018年6月的29例结直肠癌患者的癌组织和癌旁正常组织标本行RT-qPCR检测。采用χ2检验分析SPNS2在结直肠癌组织中的表达及与患者临床病理特征的关系。采用单因素和多因素分析SPNS2在结直肠癌组织中的表达情况与患者预后的关系。
      结果  免疫组织化学法结果显示SPNS2在结直肠癌组织中的表达较癌旁正常组织高（81.20% vs. 22.60%，χ2=69.136，P＜0.001）。RT-qPCR结果显示SPNS2 mRNA在结直肠癌组织中的表达水平显著高于其对应的癌旁正常组织（t=3.974，P＜0.001）。SPNS2蛋白过表达与肿瘤分化程度、淋巴结转移数、肿瘤大小和浸润深度密切相关，差异具有统计学意义（P＜0.001）。SPNS2阴性患者的生存期明显优于阳性患者（χ2=13.080，P＜0.001）。单因素分析和Cox多因素分析表明，SPNS2异常表达会影响结直肠癌患者总生存率（overall survival，OS），是患者预后的独立影响因素（P＜0.05）。
      结论  SPNS2在结直肠癌组织中的阳性表达与结直肠肿瘤的发生发展有关，SPNS2作为一种新型结直肠肿瘤标记物，在临床上可作为评估患者预后、监测术后肿瘤侵袭转移的指标及诊疗新靶点。

     

    Abstract:
      Objective  To investigate the expression and clinical significance of sphingosine-1-phosphate transporter 2 (spinster homolog 2, SPNS2) in colorectal cancer.
      Methods  Colorectal cancer and adjacent normal tissues were collected from 133 patients undergoing colorectal cancer resection in Anhui Provincial Hospital from January 2010 to June 2013. The expression of SPNS2 protein was detected by immunohistochemistry. RT-qPCR was performed on the cancer tissues and adjacent normal tissues of 29 patients with colorectal cancer from February 2018 to June 2018. The relationship between the expression of SPNS2 in cancer tissues and clinicopathological features of colorectal cancer patients was analyzed using χ2 test. The relationship between the expression of SPNS2 and prognosis of patients with colorectal cancer was analyzed based on univariate and multivariate analysis.
      Results  Immunohistochemistry results indicated that the expression of SPNS2 in colorectal cancer tissues was higher than that in adjacent normal tissues (81.20% vs. 22.60%, χ2=69.136, P < 0.001). The results of RT-qPCR indicated that the expression of SPNS2 mRNA in colorectal cancer tissues was significantly higher than that in the corresponding adjacent normal tissues (t=3.974, P < 0.001). The overexpression of SPNS2 protein was closely related to tumor differentiation, lymph node metastasis (pN), tumor size, and depth of invasion (Tx); this difference was statistically significant (P < 0.001). The survival time of patients with negative SPNS2 expression was significantly longer than that of patients with positive expression (χ2=13.080, P < 0.001). Univariate and Cox multivariate analyses showed that abnormal expression of SPNS2 affects the overall survival of colorectal cancer patients and is an independent influencing factor for patient prognosis.
      Conclusions  The positive expression of SPNS2 in colorectal cancer is related to the occurrence and development of colorectal cancer. SPNS2 can be used as a new colorectal tumor marker to evaluate the prognosis of patients, monitor the invasion and metastasis of postoperative tumors, and serve as a new target for diagnosis and treatment.

     

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