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摘要:
目的 探讨驱动蛋白家族成员20A（kinesin family member 20A，KIF20A）在大肠癌的表达及其与预后的关系。 方法 分析TCGA数据库文献中KIF20A在大肠癌组织及癌旁正常组织中mRNA的表达；收集2011年1月至2012年12月在河南大学淮河医院105例经术后病理学检测确诊为大肠癌的石蜡组织样本，采用免疫组织化学法检测KIF20A在大肠癌组织中的蛋白表达，并分析KIF20A与临床病理参数及预后的相关性。 结果 TCGA数据库分析结果表明，KIF20A在大肠癌组织中高表达，在癌旁正常组织中表达阴性或低表达（P < 0.001）；免疫组织化学法检测表明KIF20A在105例大肠癌组织中的阳性及阴性表达率分别为64%（67/105）和36%（38/105），差异具有统计学意义（P < 0.05）。KIF20A高表达与肿瘤浸润深度、淋巴结转移、远处转移、TNM分期有显著相关性（P < 0.05）。Kaplan-Meier生存分析显示，KIF20A高表达的患者生存时间、无复发生存时间显著缩短（P < 0.001）。Cox回归分析显示KIF20A是影响大肠癌患者预后的独立危险因素。 结论 KIF20A在大肠癌中表达上调，其可能作为预测大肠癌患者预后的分子标志物，参与大肠癌的发生发展过程。 Abstract:Objective To explore the expression of KIF20A (kinesin family member 20A) in colorectal cancer (CRC) tissues and adjacent normal tissues, and to analyze the relationship between KIF20A expression level and clinicopathological factors in CRC patients. Methods Data from The Cancer Genome Atlas (TCGA) database were used to analyze KIF20A mRNA expression in CRC tissues and adjacent normal tissues. A total of 105 paraffin samples were obtained from CRC patients who had undergone surgery at Huai He Hospital of Henan University, from January 2011 to December 2012. Immunohistochemical staining (IHC) was performed to examine KIF20A protein expression in tumor samples for which complete clinical and pathological data were available. Statistical analyses were applied to analyze the association between KIF20A expression and the clinical data, as well as with survival outcomes. Results Bioinformatics analysis showed that the mRNA expression level of KIF20A was upregulated in CRC tissues and normal tissues (P < 0.001). IHC revealed significantly higher expression of KIF20A in CRC tissues from 67 patients (64%) and lower or undetectable expression in 38 patients (36%). The difference was statistically significant (P < 0.05). Overexpression of KIF20A in CRC tissues was significantly associated with depth of invasion, lymphatic node metastasis, distant metastasis, and TNM stage (all P < 0.05). Kaplan-Meier survival analysis showed that patients with high levels of KIF20A expression had poor prognosis compared to patients with low levels of KIF20A expression. Cox proportional hazard regression analysis revealed that KIF20A was an independent prognostic factor in patients with CRC. Conclusions KIF20A is upregulated in CRC tissues and could serve as a novel prognostic biomarker for CRC patients. -
Key words:
- KIF20A /
- colorectal cancer /
- prognosis
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图 1 大肠癌与癌旁正常组织中KIF20A差异表达
A：TCGA数据库中，KIF20A在非配对大肠癌组织（n=380）及癌旁正常组织（n=50）中的mRNA表达水平；B：TCGA数据库中，KIF20A在配对大肠癌组织及癌旁正常组织（n=32）中的mRNA表达水平
图 2 免疫组织化学法分析KIF20A在大肠癌组织与癌旁正常组织中的蛋白表达水平及其与预后的关系
A：KIF20A在大肠癌组织中的表达（×100，×200）；B：KIF20A在癌旁正常组织中的表达（×100，×200）；C：Kaplan-Meier分析KIF20A表达对患者总体生存期的影响；D：Kaplan-Meier分析KIF20A表达对患者无复发生存期的影响
表 1 105例大肠癌患者临床病理参数与KIF20A表达的相关性
表 2 总体生存期的单因素和多因素Cox回归分析
表 3 无复发生存期的单因素和多因素Cox回归分析
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