Abstract:
Objective To assess the expression of ajuba LIM protein (AJUBA) in oral squamous cell carcinoma (OSCC) and to determine its role in cell proliferation, migration, and invasion in OSCC.
Methods The expression of AJUBA at mRNA and protein levels in OSCC was determined by quantitative polymerase chain reaction (qPCR) and immunohistochemical approaches, respectively. This was followed by analysis of the correlations between AJUBA levels and clinicopathological features of OSCC. The effects of AJUBA on cell proliferation, migration, and invasion in OSCC were assessed by MTT, wound healing, and transwell migration assays, respectively. Western blot assays were performed to check for the potential regulation of the Snail/E-cadherin pathway by AJUBA.
Results The expression of AJUBA was significantly higher in OSCC tissues compared to that in adjacent normal tissues and correlated with T stage, cell differentiation, lymph node metastasis, and recurrence in OSCC. Elevated AJUBA levels indicated poor prognosis in patients with OSCC. Depletion of AJUBA impaired cell proliferation, migration, and invasion abilities of OSCC cells. Data from Western blot assays showed that AJUBA facilitated the expression of Snail but inhibited that of E-cadherin.
Conclusions AJUBA is overexpressed in OSCC and may influence cell proliferation and invasion in OSCC by modulating the Snail/E-cadherin pathway.