曹长琦, 常琳, 吴齐. SEPT9与RNF180基因甲基化检测在早期胃癌筛查中的诊断价值[J]. 中国肿瘤临床, 2019, 46(24): 1251-1255. DOI: 10.3969/j.issn.1000-8179.2019.24.370
引用本文: 曹长琦, 常琳, 吴齐. SEPT9与RNF180基因甲基化检测在早期胃癌筛查中的诊断价值[J]. 中国肿瘤临床, 2019, 46(24): 1251-1255. DOI: 10.3969/j.issn.1000-8179.2019.24.370
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Cao Changqi, Chang Lin, Wu Qi. Circulating methylated SEPT9 and RNF180 for noninvasive diagnosis of early gastric cancer[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2019, 46(24): 1251-1255. DOI: 10.3969/j.issn.1000-8179.2019.24.370
Citation: Cao Changqi, Chang Lin, Wu Qi. Circulating methylated SEPT9 and RNF180 for noninvasive diagnosis of early gastric cancer[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2019, 46(24): 1251-1255. DOI: 10.3969/j.issn.1000-8179.2019.24.370
shu

SEPT9与RNF180基因甲基化检测在早期胃癌筛查中的诊断价值

Circulating methylated SEPT9 and RNF180 for noninvasive diagnosis of early gastric cancer

    摘要
  • 摘要:
      目的  胃癌在恶性肿瘤死亡率排名中位居前列，由于诊断时通常为进展期，因此预后较差。无创的早期胃癌（early gastric cancer，EGC）筛查技术，对提高患者5年生存率、降低癌症相关死亡率非常重要。本研究探讨外周血中SEPT9和RNF180的甲基化对EGC的诊断价值。
      方法  本研究共纳入74例EGC患者（EGC组），99例胃良性疾病患者（胃良性疾病组），包括炎症、息肉、肠上皮化生、胃良性溃疡等，以及57例健康受试者（健康对照组）。对上述3组患者外周血中SEPT9和RNF180的甲基化进行检测，计算敏感度、特异度、阳性预测值和阴性预测值，并计算ROC曲线下面积（AUC）。
      结果  SEPT9甲基化对EGC的敏感度为28.3%（95% CI：18.5%~40.0%），特异度为94.2%（95% CI：89.3%~97.3%），AUC为0.616（95% CI：52.0%~71.1%）。RNF180甲基化的敏感度为32.4%（95% CI：22.0%~44.3%），特异度89.7%（95% CI：83.9%~94.0%），AUC为0.636（95% CI：54.2%~73.0%）。两个基因甲基化的联合诊断敏感度为40.5%（95% CI：29.3%~52.6%），特异度为85.3%（95% CI：78.7%~90.4%），AUC为0.650（95% CI：55.7%~74.4%）。
      结论  SEPT9和RNF180甲基化有可能作为诊断EGC的生物学标志物。

     

    Abstract:
      Objective  Gastric cancer is a common malignancy with poor prognosis owing to its frequent diagnosis at advanced stages when metastasis has already occurred. To improve the 5-year survival rate and reduce the number of cancer-related deaths in patients with gastric cancer, it is necessary to develop noninvasive methods for early detection. This study aimed to evaluate the diagnostic value of circulating methylated SEPT9 (mSEPT9) and ring finger protein 180 (mRNF180) in patients with early gastric cancer.
      Methods  We enrolled 74 patients with early gastric cancer, 99 with benign gastric diseases (inflammation, polyps, intestinal metaplasia, ulcers, and erosion), and 57 healthy controls. Methylation of SEPT9 and RNF180 genes was detected in each group, and positivity rates were calculated. We determined the sensitivity, specificity, positive predictive value, negative predictive value, confidence interval (CI), and area under the curve (AUC) for methylation of these genes in patients with early gastric cancer.
      Results  As a diagnostic target, SEPT9 methylation had a sensitivity of 28.3% (95% CI:18.5~40.0%), specificity of 94.2% (95% CI:89.3~97.3%), and an AUC value of 0.616 (95% CI:52.0~71.1%). RNF180 methylation had a sensitivity of 32.4% (95% CI:22.0~44.3%), specificity of 89.7% (95% CI:83.9~94.0%), and an AUC value of 0.636 (95% CI:54.2~73.0%). A combination of the two targets yielded a sensitivity of 40.5% (95% CI:29.3~52.6%), specificity of 85.3% (95% CI:78.7~90.4%), and an AUC value of 0.65 (95% CI:55.7~74.4%).
      Conclusions  SEPT9 and RNF180 methylation could be used as diagnostic biomarkers for detecting early gastric cancer.

     

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