郭晓锋, 朱曼, 许广辉, 王文光, 郝安林. 长链非编码RNA SNHG3在食管鳞状细胞癌中的表达及其对ECA-109细胞迁移和侵袭的影响[J]. 中国肿瘤临床, 2020, 47(12): 595-600. DOI: 10.3969/j.issn.1000-8179.2020.12.413
引用本文: 郭晓锋, 朱曼, 许广辉, 王文光, 郝安林. 长链非编码RNA SNHG3在食管鳞状细胞癌中的表达及其对ECA-109细胞迁移和侵袭的影响[J]. 中国肿瘤临床, 2020, 47(12): 595-600. DOI: 10.3969/j.issn.1000-8179.2020.12.413
shu
Xiaofeng Guo, Man Zhu, Guanghui Xu, Wenguang Wang, Anlin Hao. Expression of long non-coding RNA SNHG3 in esophageal squamous cell carcinoma and its effect on the migration and invasion of ECA-109 cells[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2020, 47(12): 595-600. DOI: 10.3969/j.issn.1000-8179.2020.12.413
Citation: Xiaofeng Guo, Man Zhu, Guanghui Xu, Wenguang Wang, Anlin Hao. Expression of long non-coding RNA SNHG3 in esophageal squamous cell carcinoma and its effect on the migration and invasion of ECA-109 cells[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2020, 47(12): 595-600. DOI: 10.3969/j.issn.1000-8179.2020.12.413
shu

长链非编码RNA SNHG3在食管鳞状细胞癌中的表达及其对ECA-109细胞迁移和侵袭的影响

Expression of long non-coding RNA SNHG3 in esophageal squamous cell carcinoma and its effect on the migration and invasion of ECA-109 cells

    摘要
  • 摘要:
      目的  探究长链非编码RNA SNHG3在食管鳞状细胞癌（esophageal squamous cell carcinoma，ESCC）组织中的表达及其对ECA-109细胞迁移和侵袭的影响。
      方法  收集安阳市肿瘤医院2011年6月到2014年6月收治的60例ESCC患者癌及对应癌旁组织样本，采用qRT-PCR检测ESCC癌与癌旁组织、ESCC细胞ECA-109和人正常食管上皮细胞HEEC中lncRNA SNHG3的表达水平，分析ESCC患者组织中lncRNA SNHG3的表达与ESCC患者临床特征的关系。采用siRNA-SNHG3质粒转染ECA-109细胞，以敲降lncRNA SNHG3的表达水平。采用Kaplan-Meier法分析lncRNA SNHG3表达与患者预后的关系。采用Transwell实验检测ECA-109细胞的迁移和侵袭能力。采用qRT-PCR和Western blot实验检测ECA-109细胞中SNAIL和TWIST的mRNA及蛋白的表达水平。
      结果  ESCC肿瘤组织中lncRNA SNHG3的表达水平显著高于癌旁组织（P < 0.05），ECA-109细胞中lncRNA SNHG3的表达水平显著高于人正常食管上皮细胞HEEC（P < 0.05）。ESCC患者组织中lncRNA SNHG3的表达水平与肿瘤分化程度、TNM分期及淋巴结转移情况显著相关（P < 0.05）。siRNA-SNHG3质粒转染ECA-109细胞后，lncRNA SNHG3的表达水平显著降低（P < 0.05）。lncRNA SNHG3的高表达与ESCC患者预后不良显著相关。敲降lncRNA SNHG3后，ECA-109细胞的迁移和侵袭能力显著降低（P < 0.05），SNAIL和TWIST的mRNA及蛋白的表达水平显著降低（P < 0.05）。
      结论  lncRNA SNHG3在ESCC肿瘤组织和细胞中高表达，通过调控SNAIL和TWIST蛋白的表达，促进ECA-109细胞的迁移和侵袭。

     

    Abstract:
      Objective  To investigate the expression of long non-coding RNA(lncRNA) SNHG3 in esophageal squamous cell carcinoma (ESCC) and its effect on the migration and invasion of ECA-109 cells.
      Methods  Samples of tumor and corresponding para-carcinoma tissues were collected from 60 patients with ESCC, who were enrolled in Anyang Tumor Hospital from June 2011 to June 2014. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of lncRNA SNHG3 in ESCC tumor and para-carcinoma tissues, ECA-109 ESCC cells, and HEEC human normal esophageal epithelial cells. The correlation between lncRNA SNHG3 expression and clinical characteristics of patients with ESCC was assessed. ECA-109 cells were transfected with siRNA-SNHG3 plasmids to knockdown lncRNA SNHG3 expression. The correlation between lncRNA SNHG3 expression and patient prognosis was determined by Kaplan-Meier analysis. Transwell assay was used to investigate the migration and invasion abilities of ECA-109 cells. SNAIL and TWIST mRNA and protein levels in ECA-109 cells were detected by qRT-PCR and Western blot assay, respectively.
      Results  The expression level of lncRNA SNHG3 was significantly higher in ESCC tumor tissues compared to that in pericarcinomatous tissues and in ECA-109 cells compared to that in HEEC cells (both P < 0.05). LncRNA SNHG3 expression in ESCC cells was significantly correlated with tumor differentiation, TNM stage, and lymph node metastasis (P < 0.05). LncRNA SNHG3 expression in ECA-109 cells decreased significantly upon transfection with the siRNA-SNHG3 plasmid (P < 0.05). High expression of lncRNA SNHG3 was significantly correlated with poor prognosis in patients with ESCC. After lncRNA SNHG3 knockdown, the migration and invasion of ECA-109 cells and the mRNA and protein expression levels of SNAIL and TWIST were reduced significantly (P < 0.05).
      Conclusion  LncRNA SNHG3 is highly expressed in ESCC tumor tissues and cells and promotes the migration and invasion of ECA-109 cells by regulating the expression of SNAIL and TWIST proteins.

     

    • HTML全文
    • 参考文献(26)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回