Abstract:
Objective To investigate the relationship between mutated epidermal growth factor receptor (EGFR) and the expression of G-protein coupled estrogen receptor 1 (GPER1)/GPR30 in lung adenocarcinoma.
Methods The expression of GPER1 in 83 cases of lung adenocarcinoma after surgery was examined using immunohistochemistry and EGFR gene mutation status in the corresponding tis-sues were detected using second-generation gene sequencing. At the same time, clinical pathological data of the patients were collect-ed and the correlation between GPER1 and EGFR was analyzed. In addition, the expression level of GPER1 in lung adenocarcinoma A549 cells with EGFR wild type, PC-9 cells with EGFR mutation, and PC-9 cells treated with gefitinib were examined by Western blot.
Results The positive expression of GPER1 was not correlated with gender, age, tumor size, smoking, and degree of differentiation (P > 0.05) and EGFR mutations were not correlated with tumor TNM staging (P=0.542). Positive GPER1 expression was positively correlated with EGFR mutations (P=0.003) and GPER1 expression was higher in stage Ⅲ and Ⅳ lung adenocarcinoma than in stage Ⅰ and Ⅱ (P= 0.008). In addition, the expression of GPER1 decreased when EGFR signaling pathway was inhibited by gefitinib in PC-9 cells.
Conclusions The expression of GPER1 in EGFR- mutated lung adenocarcinoma is higher than that of EGFR wild type lung adenocarcinoma, which suggested that GPER1 expression is regulated by the EGFR signaling pathway.
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