Abstract: Objective : To investigate the effect of resveratrol on the expression of epidermal growth factor (EGF) and to identify the effect of resveratrol on the invasion of cervical cancer in vitro. Methods : The effect of resveratrol on invasive ability of HeLa cells was observed with a Transwell cell culture chamber. Changes in parapodium structure were observed with scanning electron microscopy. EGF mRNA and protein expression in HeLa cells was detected by RT-PCR and Western blot. Results : In the Transwell cell culture chambers, the average number of invading HeLa cells was 9.20±1.924 in the group with 1mM resveratrol and 10.20±2.049 in the group with 0.5mM resveratrol, significantly lower than those in the control group and the DMSO group (39.80± 2.387, 39.00±3.808, respectively) (P <0.05). The levels of EGF mRNA and protein were also decreased after treatment with resveratrol. The parapodium structures of cells cultured with resveratrol appeared to be broken down. Conclusion : Resveratrol can effectively decrease the expression of EGF and then destroy the parapodium structures of cervical cancer cells. Through this mechanism, resveratrol can inhibit the migration and invasion of cervical cancer cells in vitro.