nm23 VEGF-C及VEGFR-3在大肠癌中的表达及意义
Expression and Clinical Significance of nm23, VEGF-C and VEGFR-3 in Colorectal Carcinoma
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摘要: 目的: 探讨nm23、VEGF-C及其受体VEGFR-3在大肠癌中的表达和三者间的相互关系,及其在大肠癌淋巴结转移和肿瘤进展中的意义。 方法: 采用免疫组化SP法检测97例大肠癌组织和97例正常大肠组织中nm23、VEGF-C及VEGFR-3的表达。 结果: 1)A、B期大肠癌及无淋巴结转移的大肠癌组织中nm23的阳性表达率分别为68.9%、68.2%,C、D期及有淋巴结转移的大肠癌组织中nm23阳性表达率分别为30.6%、25.8%,A、B期高于C、D期,无淋巴结转移高于有淋巴结转移,差别有统计学意义(P<0.05);VEGF-C阳性表达率在C、D期及有淋巴结转移的大肠癌组织中分别为75.0%、77.4%,在A、B期及无淋巴结转移的大肠癌组织中分别为49.2%、50.0%,两者比较,差别有统计学意义(P<0.05);VEGFR-3在C、D期及有淋巴结转移的大肠癌中的阳性表达率分别为63.9%、67.7%,在A、B期及无淋巴结转移的大肠癌组织中分别为41.0%、40.9%,差别有统计学意义(P<0.05。2)nm23与分化程度、肠壁侵犯深度、有无淋巴结转移及Duckes分期有关(P<0.05),VEGF-C及VEGFR-3与分化程度、淋巴结转移及Duckes分期有关(P<0.05)。3)nm23在正常大肠组织和大肠癌组织中的阳性表达率分别为83.5%、54.6%,差别有统计学意义(P<0.05),VEGF-C在正常大肠组织和大肠癌组织中的阳性表达率分别为29.9%、58.8%,差别有统计学意义(P<0.05),VEGFR-3在正常大肠组织和大肠癌组织中的阳性表达率分别为21.6%、49.5%,两者比较,差别有统计学意义(P<0.05)。 结论: nm23与VEGF-C和VEGFR-3在大肠癌中的表达呈负相关关系,nm23基因对大肠癌有抑制作用,VEGF-C和VEGFR-3与大肠癌的侵袭和转移密切相关,三者的联合检测可作为评价大肠癌病情、推测预后及指导治疗的重要参考指标。Abstract: Objective :To investigate the expression of nm23,vascular endothelial growth factor C (VEGF-C) andvascular endothelial growth factor receptor 3 (VEGFR-3) in tissues of colorectal carcinoma and to explore thesignificance of their roles in lymph node metastasis and progression of colorectal carcinoma. Methods :Expres-sion of nm23,VEGF-C and VEGFR-3 were detected in 97 tissue samples of colorectal carcinoma and 97 tis-sue samples of corresponding normal mucosa with immunohistochemistry. Results :The expression of nm23 was higher in colorectal carcinoma of stages A and B than in stages C and D,and it was higher in colorectalcarcinoma without lymph node metastasis than in colorectal carcinoma with lymph node metastasis (P<0.05).The expression of VEGF-C and VEGFR-3 was higher in colorectal carcinoma of stages C and D than in col-orectal carcinoma of stages A and B,and the expression of both proteins was higher in colorectal carcinomawith lymph node metastasis than in colorectal carcinoma without lymph node metastasis (P<0.05).Expressionof nm23 was related to the degree of differentiation,depth of invasion,lymph node metastasis and Duke'sstage (P<0.05).The expression of VEGF-C and VEGFR-3 was correlated with the degree of differentiation,lymph node metastasis and Duke's stage (P<0.05).Significant differences were found in the positive rates ofnm23,VEGF-C and VEGFR-3 expression between normal large intestine tissues and tissues of colorectal car-cinoma (P<0.05). Conclusion :The expression of nm23 was negatively correlated with the expression ofVEGF-C and VEGFR-3 in colorectal carcinoma.Combined analysis of nm23,VEGF-C and VEGFR-3 can pro-vide valuable evidence for diagnosis of colorectal carcinoma,estimation of its prognosis,and guidance toward the optimal clinical treatment.
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