Abstract: Objective : To study the effect of Hepatitis B Virus X protein (HBx) on the expression of β-catenin in theL02 normal human hepatocyte cell line. Methods : A recombinant adenovirus expressing the HBx gene alongwith GFP was used to transfect the L02 normal human hepatocyte cell line. The expression of HBx protein in L02 cells was evaluated by Western blot. RT-PCR and Western blot were used to assess the effect of HBx onthe expression of β-catenin in the L02 cell line. Results : A high titer of the recombinant adenovirus expressingHBx was obtained, and the virus was effectively transfected into L02 cells. HBx expression induced an in-crease in the expression of β-catenin in transfected L02 cells. Conclusion : The up-regulation of β-catenin inthe L02 cell line indicates that HBx could activate the Wnt signaling pathway by regulating the ectopic expres-sion of β-catenin. HBx may play a role in regulating the expression of β-catenin which is important in the de-velopment of HCC.