Abstract: Objective : To investigate the clinical significance of TuM2-PK detection in the diagnosis of cholangiocarci-noma. Methods : From January 2005 to November 2007, the TuM2-PK and CA19-9 tumor markers were de-tected by ELISA in 120 patients with cholangiocarcinoma and 170 controls (120 healthy cases). Results : Thelevels of TuM2-PK and CA19-9 were remarkably higher in patients with cholangiocarcinoma than in the con-trols (P<0.05). TuM2-PK showed higher sensitivity (85%) and specificity (88%) than CA19-9 (67% and 72%,respectively). In the combined test, the sensitivity and specificity were 95% and 96%. No significant differencewas found among patients with various locations of cholangiocarcinoma or various pathological types ofcholangiocarcinoma. A significant difference was found in the concentration of TuM2-PK between stage Ⅰ/Ⅱand stage Ⅲ/Ⅳ cholangiocarcinoma (P<0.05). The sensitivity and specificity of TuM2-PK detection for stageⅢ/Ⅳ cholangiocarcinoma were significantly higher than those for stage Ⅰ/Ⅱ cholangiocarcinoma (P<0.05). Conclusion : TuM2-PK can be used as a valuable marker for the diagnosis of cholangiocarcinoma and CA19-9can elevate the sensitivity and specificity. TuM2-PK can also be an index for preoperative clinical staging ofcholangiocarcinoma.