Abstract: Objective: To investigate the expression and clinical significance of uPARAP/Endo180 (uPAR-associated protein),urokinase plasminogen activator receptor(uPAR) and vascular endothelial growth factor(VEGF) in nasopharyngeal carcino-ma(NPC). Methods: Streptavidin-horseradish peroxidase immunohistochemistry (SP-IHC) method was used to detect the expression of uPARAP, uPAR and VEGF in 58 samples of NPC and 30 samples of chronic nasopharynxitis. Results: The positive expression of uPARAP in NPC was mainly confined to mesenchymal cells, including fibroblasts and macrophages.The uPARAP immunoreactivity was found as granulated diffuse cytoplasmic staining, as well as in a pattern suggesting lo-calization on the cell surface. uPAR in NPC tissues was evenly distributed in the cytoplasm of both cancer cells and mes-enchymal cells. The expression of VEGF in NPC was mainly seen in the cytoplasm of tumor cells. In the NPC tissues, the expression rate of uPARAP, uPAR and VEGF was 75.9%, 81.0%, and 77.6%, respectively (P<0.05). A significant differ-ence was found in the expression of uPARAP, uPAR and VEGF between NPC tissues and chronic nasopharynxitis tissues.The expression of uPARAP was positively correlated with the expression of uPAR and VEGF in NPC. The positive expres-sion rate of uPARAP was 42.9% in keratinizing NPC, 66.7% in non-keratinizing NPC, and 86.1% in undifferentiated car-cinoma. The expression of uPARAP was associated with the differentiation of NPC. The expression of uPARAP was signif-icantly different among different pathological types of NPC(P<0.05). The expression of uPARAP in nasopharyngeal carci-noma was significantly correlated with pathological classification, tumor grade, cervical lymph node metastases and clinical staging, but not with age or gender. Conclusion: Co-expression of uPARAP, uPAR and VEGF may be important for metastasis of NPC. Combined detection of these proteins may serve as a useful biomarker for evaluating the potential of metastasis and prognosis of NPC.