李小丽, 司同国, 于海鹏, 郭志. 冷冻消融对肝癌患者外周血CD4+CD25+调节T细胞影响的初步研究[J]. 中国肿瘤临床, 2008, 35(8): 453-454.
引用本文: 李小丽, 司同国, 于海鹏, 郭志. 冷冻消融对肝癌患者外周血CD4+CD25+调节T细胞影响的初步研究[J]. 中国肿瘤临床, 2008, 35(8): 453-454.
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LI Xiaoli, SI Tongguo, YU Haipeng, GUO Zhi. Effect of Cryosurgery on Regulatory CD4+CD25+ T Lymphocytes in the Peripheral Blood of Patients with Hepatocellular Carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2008, 35(8): 453-454.
Citation: LI Xiaoli, SI Tongguo, YU Haipeng, GUO Zhi. Effect of Cryosurgery on Regulatory CD4+CD25+ T Lymphocytes in the Peripheral Blood of Patients with Hepatocellular Carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2008, 35(8): 453-454.
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冷冻消融对肝癌患者外周血CD4+CD25+调节T细胞影响的初步研究

Effect of Cryosurgery on Regulatory CD4+CD25+ T Lymphocytes in the Peripheral Blood of Patients with Hepatocellular Carcinoma

    摘要
  • 摘要: 目的: 探讨氩氦冷冻对肝癌外周血CD4+CD25+调节T细胞的影响及临床意义。 方法: 原发性肝癌患者20例,其中Ⅱ期14例、Ⅲ期6例,分别采集氩氦冷冻治疗术前及术后1个月外周血,采用流式细胞术检测外周血T淋巴细胞亚群(CD3+T、CD4+T、CD8+T、CD4+T/CD8+T、NK细胞)及Treg细胞。 结果: 肝癌Ⅲ期患者外周血Treg细胞占CD4+T细胞比例明显高于Ⅱ期患者(6.5%±1.2%、9.1%±2.0%,P=0.013)。与氩氦冷冻治疗前比较治疗后1个月Treg细胞一定程度降低(8.2%±1.1%、7.7%±1.0%),但差异无显著性(P=0.052);CD4+T、CD4+T/CD8+T、NK细胞明显升高,CD8+T细胞降低(P<0.05)。 结论: 肿瘤负荷可显著促进肝癌患者外周血Treg细胞分化。肝癌氩氦冷冻治疗后短期内Treg细胞比例轻度下降,T淋巴细胞亚群分布异常得到一定改善。

     

    Abstract: Objective: To analyze the effect of Argon-Helium Cryosurgery (AHCS) on the differentiation of regulatory CD4+CD25+ T cell(Treg) and its implication in patients with hepatocellular carcinoma(HCC). Methods: Peripheral venous blood samples were obtained from 20 patients with HCC before and 1 month after AHCS. Treg cells and T lymphocyte subsets in the peripheral blood were measured by flow cytometry. Results: The percentage of Treg cells in stage III patients were higher than in stage II patients (P<0.05). After AHCS, the percentage of Treg cells was decreased, but with no significant difference(P>0.05). CD4+T, NK and CD4+T/CD8+T were significantly increased after AHCS(P<0.05). Conclusion: Tumor load can significantly promote the differentiation of Treg cells in patients with HCC. After the therapy of AHCS, the per-centage of Treg has no significant changes in a short time. The abnormal distribution of subsets of T-lymphocytes can be improved.

     

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