Abstract: Objective : To study the effects and the mechanism of different tissue extracts on the invasive and metastatic ability of gastric carcinoma. Methods : Using the Trans well invasion assay, we observed the influ-ence of different tissue extracts on the invasive ability of GASL cells in vitro. Gelatin zymography was used todetect the secretion and activity of Gelatinase in GASL cells incubated with different tissue extracts. The adhe-sive property of GASL to the different tissue extracts was measured by MTT assay. Results : Trans well inva-sion assay showed that the numbers of cells incubated with lung, liver, pancreas, spleen and brain extracts were (10.2±3.1), (21.3±2.3), (25.4±4.1), (9.3±1.6), (6.0±0.8), and (7.2±1.1), respectively. In comparison to the control group and other tissue extracts, the tissue extracts of liver and pancreas promoted the invasive abilityof GASL cells ( P <0.05). After incubation with extracts of liver or pancreas, the secretions of MMP-9 and MMP-2 were up-regulated in GASL cells. The GASL cells showed a higher coefficient of adhesion to the liver and pancreas tissue extract than to the other tissue extracts ( P <0.05). Conclusion : With a higher coefficient ofadhesive property, the liver and pancreas tissue extracts can promote the invasive ability of GASL cell sthrough stimulating the secretion of Gelatinase.