Abstract: Objective: Previous studies have shown that a downregulation of kinesin-like 4 (KNSL4) mRNA level is correlated with poor prognosis in breast cancer patients.The purpose of this study was to identify the target genes of kinesin-like DNA binding protein (Kid) and to investigate the role of Kid in the carcinogenesis and development of breast cancer. Methods: DNA sequences occupied by Kid in MDA-MB-435S cells were profiled based on the chromatin immunoprecipitation-DNA selection and ligation (ChIP-DSL) method combined with promoter microarray experiments.The genes that were 2-fold or more differentially enriched by anti-Kid antibody compared to non-antibody were considered to be candidate genes.The candidate genes were validated by ChIP-PCR assay.The expression of candidate genes in MDA-MB-435S cells and MDA-MB-435S/KNSL4-siRNA cells was detected by real-time reverse transcription-PCR (RT-PCR) assay. Results: A total of 287 candidate genes were considered after ChIP-DSL and promoter microarray.ChIP-PCR assay showed that ATF7IP and CDC25C genes were 2.2 and 2.4-fold enriched, respectively, by anti-Kid antibody.The expres-sion of ATF7IP and CDC25C mRNA in MDA-MB-435S/KNSL4-siRNA cells was 1.6 and 5.8-fold upregulated compared with the parental cells. Conclusion: Kid negatively regulates transcription of the ATF7IP and CDC25C genes, and it may be involved in the carcinogenesis and development of breast cancer through this mechanism.