Abstract: Objective: To investigate the anti-tumor, anti-angiogenic and anti-metastatic effect of endostatin on Lewis lung carcino-ma xenograft in mice. Methods: Different dosages of Endostatin and Cisplatin were given to C57 mice with Lewis lung carcinoma xenograft.Tumor growth, VEGF expression, MVD in planted xenogrft tumor and metastatic tumor, and the metastatic rate were then observed and analyzed. Results: Endostatin obviously inhibited Leweis tumor growth.A signifi-cant difference was found in tumor growth between the group treated with Endostatin and the control group (P=0.0303).Endostatin remarkably down-regulated VEGF expression both in xenograft and metastastic tumor tissues.A positive cor-relation was found between VEGF expression in the xenograft and that in lung metastastic tumor tissues (r=0.977, P= 0.001).Endostatin decreased MVD both in xenograft and metastastic tumor tissues.Tumors treated with Endostatin had less lung metastases, the extent of which depended on the dosage. Conclusion: Endostatin can inhibit the growth and metastases of Lewis lung carcinoma through down-regulating VEGF expression and MVD both in the xenograft and metastatic tumors.