Abstract: Objective : To evaluate the feasibility of intraoperative detection of sentinel lymph nodes (SLN) in patients with endometrial cancer. Methods : Thirty-one patients with clinical stage I-II endometrial cancer treated with hysterectomy and lymph node excision were enrolled in the study.At laparotomy, methylene blue dye was in-jected into the subserosal myometrium of corpus uteri at multiple sites.Dye uptake into lymphatic channels was observed and blue nodes identified as SLNs were excised.Other nodes were also removed.All resected nodes were submitted to pathology after hematoxylin and eosin (H&E) staining. Results : Dye uptake failed in 4 (12.9%) cases because of spillage.Blue dye diffusing to the lymphatic channels in the uterine surface and infundibulopelvic ligaments was seen in the remaining 27 (87.1%) cases.Among those cases, blue dye taken up into pelvic lymphatic vessels was observed in 22 patients.The SLNs were identified in 23 of the 27 (85.2%) cases.A total of 90 SLNs with a mean number of 3.9 SLNs (range, 1-10) for each patient were identi-fied.One SLN was found in the paraaortic area, and the other 89 SLNs (98.9%) were in the pelvis.The most frequent locations of SLNs included obturator in 38 patients (42.2%) and interiliac lymph nodes in 19 patients (21.1%).Twenty seven (87.1%) patients received pelvic lymphadenectomy and 4 received pelvic node sam-pling.Of the 31 cases, 7 received paraaortic node sampling.A total of 926 nodes with a mean number of 39.8 nodes (range: 14-55) for each patient were harvested.Three patients (9.7%) had nodal metastases, 2 of them had involvement with at least 1 SLN, and one patient had no SLN.No adverse reactions or injuries were at-tributed to the study. Conclusion : Intraoperative SLN identification with methylene blue dye in endometrial can-cer is feasible and safe.