Abstract: Objective : To study the inhibitory effects of Ad-ODC-AdoMetDCas on polyamine biosynthesis, growth and apoptosis of esophageal cancer cells. Methods : Adenovirus-mediated gene transduction efficiency wasassessed by counting GFP-positive cells using MTT. The malignant phenotype of Eca109 cells was assessedby growth curve. Western blot and HPLC were used to detect ODC and AdoMetDC expression and polyaminecontent in Eca109 cells. Viable cell cycle analysis was employed to evaluate cell cycle distribution. TUNELwas used to assess apoptosis. Results : Approximate 70% of Eca109 cells were infected with Ad-ODC-AdoMet-DCas when an MOI of 50 was used. The expression of ODC was inhibited in the infected tumor cells.Ad-ODC-AdoMetDCas inhibited Eca109 cell growth and invasive ability. The tumor cells were arrested in G 1phase after gene transfer. TUNEL analysis showed that Ad-ODC-AdoMetDCas induced cell apoptosis. Con-clusion : Ad-ODC-AdoMetDCas has significant inhibitory effects on esophageal cancer cell proliferation andthus has a therapeutic potential for the treatment of esophageal cancer.