Abstract: Objective : To investigate the effects of psychological stress and anti-anxiety drugs on tumor dormancy,relapse and metastasis. Methods : Isolation-stress and stochastic empty bottle were combined to establishmice model of chronic emotional stress, and routine anti-anxiety drug Diazepam was used to control the anxi-ety. The experimental animals included 5 groups: the dormancy control group, the anti-anxiety drug group, the group with induced anxiety, the group with induced anxiety and anti-anxiety drug, and the trauma group. Tu-mor dormant status was established by inoculation of 500 ca761-03 cells into the left leg muscle of the mice.Besides isolated caging and stochastic empty bottle, Diazepam was orally delivered twice daily in the drug group and the group with induced anxiety and anti-anxiety drug. The trauma group was given traumatic stimu-lation at the end of the first month. All animals were continuously observed for 2 months. Tissues at the site of injection of tumor cells, lungs and lymph nodes were dissected, fixed, embedded and sectioned. H&E staining was used to observe the tumor occurrence and metastasis. Results : At the end of the second month, the dor-mancy rates were 70%, 100%, 40%, 90% and 40%, respectively, in the five groups. The tumorigenecity rates were 30%, 0%, 40%, 10% and 60%, respectively. And the lung metastasis rates of each group were 20%,0%, 30%, 0% and 60%, respectively. Conclusion : Negative emotion can stimulate the breakthrough of tumordormancy and promote the growth and spread of tumor cells. Anti-anxiety drug can obviously reduce the anx-ious emotion and tumor occurrence. The anti-anxiety therapy is helpful for the maintenance of dormant status and preventing tumor relapse.