Abstract: Objective: To detect the expression and distribution of talin in colorectal carcinoma and to investigateits relationship with clinical pathological characteristics of colorectal carcinoma, including carcinogenesis, differentiation,infiltration, and metastasis. Methods: The expression of talin in 41 matched normal colorectal mucosa and primary col-orectal carcinomas and 19 regional lymph node metastases was quantitatively detected by immunofluorescence in combi-nation with confocal laser scanning microscopy. Results: Talin was located in the cytoplasm and membrane in both normalmucosa and primary carcinoma. The level of talin expression in normal colorectal mucosa was significantly higher than inprimary colorectal carcinoma, with a significant difference (P=0.000). Compared with primary colorectal carcinoma, the re-gional lymph node metastases showed lower talin expression level (P=0.000). The expression of talin in colorectal carcino-mas with lymph node metastasis was lower than in those without lymph node metastasis(P=0.000). The colorectal carcino-mas with deeper infiltration had lower talin expression than those with superficial infiltration (P=0.002). The talin expres-sion levels were not correlated with the differentiation of colorectal carcinoma (P=0.153). Conclusion: Talin is related tothe carcinogenesis, infiltration and metastasis of colorectal carcinoma but has no correlation with the differentiation of col-orectal carcinoma. The level of talin expression might be a valuable marker for diagnosis and prognosis of colorectal carci-noma.